 |
Journal articles
A yeast exosome cofactor, Mpp6, functions in RNA surveillance and in the degradation of noncoding RNA transcripts
Abstract : A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 3'―\textgreater5' exonucleases, the THO complex required for mRNP assembly, and Ynr024w (Mpp6). SL interactions with mpp6Delta were confirmed for rrp47Delta and nuclear exosome component Rrp6. The results of bioinformatic analyses revealed homology between Mpp6 and a human exosome cofactor, underlining the high conservation of the RNA surveillance system. Mpp6 is an RNA binding protein that physically associates with the exosome and was localized throughout the nucleus. The results of functional analyses demonstrated roles for Mpp6 in the surveillance of both pre-rRNA and pre-mRNAs and in the degradation of "cryptic" noncoding RNAs (ncRNAs) derived from intergenic regions and the ribosomal DNA spacer heterochromatin. Strikingly, these ncRNAs are also targeted by other exosome cofactors, including Rrp47, the TRAMP complex (which includes Air1), and the Nrd1/Nab3 complex, and are degraded by both Rrp6 and the core exosome. Heterochromatic transcripts and other ncRNAs are characterized by very rapid degradation, and we predict that functional redundancy is an important feature of ncRNA metabolism.
Keywords :
Amino Acid
Sequence Homology
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae
Untranslated
RNA Stability
Messenger
Post-Transcriptional
RNA Precursors
RNA Processing
Amino Acid Sequence
Cell Nucleus
Gene Expression Regulation
Fungal
Humans
Molecular Sequence Data
Mutation
Protein Binding
RNA-Binding Proteins
RNA
Cited literature [58 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01404695
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 10:38:49 AM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Long-term archiving on: : Monday, March 20, 2017 - 10:40:56 PM
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 10:38:49 AM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Long-term archiving on: : Monday, March 20, 2017 - 10:40:56 PM
File
Mol. Cell. Biol.-2008-Milligan...
Publisher files allowed on an open archive