Skip to Main content Skip to Navigation
Journal articles

Long open reading frame transcripts escape nonsense-mediated mRNA decay in yeast

Abstract : Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3' UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open reading frame (ORF) regions and with the same long, destabilizing 3' UTR. We developed a barcode-based DNA microarray strategy to compare the levels of each reporter mRNA in strains with or without active NMD. The size of the coding region had a significant negative effect on NMD efficiency. This effect was not specific to the tested 3' UTR because two other different NMD reporters became less sensitive to NMD when ORF length was increased. Inefficient NMD was not due to a lack of association of Upf1 to long ORF transcripts. In conclusion, in addition to a long 3' UTR, short translation length is an important feature of NMD substrates in yeast.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01404015
Contributor : Floran Bidault <>
Submitted on : Monday, November 28, 2016 - 11:31:29 AM
Last modification on : Monday, May 18, 2020 - 11:00:35 AM
Long-term archiving on: : Tuesday, March 21, 2017 - 12:38:20 AM

File

1-s2.0-S2211124714000424-main....
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Laurence Decourty, Antonia Doyen, Christophe Malabat, Emmanuel Frachon, Delphine Rispal, et al.. Long open reading frame transcripts escape nonsense-mediated mRNA decay in yeast. Cell Reports, Elsevier Inc, 2014, 6 (4), pp.593--598. ⟨10.1016/j.celrep.2014.01.025⟩. ⟨pasteur-01404015⟩

Share

Metrics

Record views

489

Files downloads

528