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Antigen persistence is required for dendritic cell licensing and CD8+ T cell cross-priming.

Résumé : It has been demonstrated that CD4(+) T cells require Ag persistence to achieve effective priming, whereas CD8(+) T cells are on "autopilot" after only a brief exposure. This finding presents a disturbing conundrum as it does not account for situations in which CD8(+) T cells require CD4(+) T cell help. We used a physiologic in vivo model to study the requirement of Ag persistence for the cross-priming of minor histocompatibility Ag-specific CD8(+) T cells. We report inefficient cross-priming in situations in which male cells are rapidly cleared. Strikingly, the failure to achieve robust CD8(+) T cell activation is not due to a problem with cross-presentation. In fact, by providing "extra help" in the form of dendritic cells (DCs) loaded with MHC class II peptide, it was possible to achieve robust activation of CD8(+) T cells. Our data suggest that the "licensing" of cross-presenting DCs does not occur during their initial encounter with CD4(+) T cells, thus accounting for the requirement for Ag persistence and suggesting that DCs make multiple interactions with CD8(+) T cells during the priming phase. These findings imply that long-lived Ag is critical for efficient vaccination protocols in which the CD8(+) T cell response is helper-dependent.
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Contributor : Marie-Christine Vougny Connect in order to contact the contributor
Submitted on : Thursday, November 24, 2016 - 3:03:58 PM
Last modification on : Thursday, April 7, 2022 - 10:10:23 AM

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Hélène Jusforgues-Saklani, Martin Uhl, Nathalie Blachère, Fabrice Lemaître, Olivier Lantz, et al.. Antigen persistence is required for dendritic cell licensing and CD8+ T cell cross-priming.. Journal of Immunology, 2008, 181 (5), pp.3067-76. ⟨10.4049/jimmunol.181.5.3067⟩. ⟨pasteur-01402307⟩



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