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Journal articles
Antigen persistence is required for dendritic cell licensing and CD8+ T cell cross-priming.
Résumé : It has been demonstrated that CD4(+) T cells require Ag persistence to achieve effective priming, whereas CD8(+) T cells are on "autopilot" after only a brief exposure. This finding presents a disturbing conundrum as it does not account for situations in which CD8(+) T cells require CD4(+) T cell help. We used a physiologic in vivo model to study the requirement of Ag persistence for the cross-priming of minor histocompatibility Ag-specific CD8(+) T cells. We report inefficient cross-priming in situations in which male cells are rapidly cleared. Strikingly, the failure to achieve robust CD8(+) T cell activation is not due to a problem with cross-presentation. In fact, by providing "extra help" in the form of dendritic cells (DCs) loaded with MHC class II peptide, it was possible to achieve robust activation of CD8(+) T cells. Our data suggest that the "licensing" of cross-presenting DCs does not occur during their initial encounter with CD4(+) T cells, thus accounting for the requirement for Ag persistence and suggesting that DCs make multiple interactions with CD8(+) T cells during the priming phase. These findings imply that long-lived Ag is critical for efficient vaccination protocols in which the CD8(+) T cell response is helper-dependent.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01402307
Contributor : Marie-Christine Vougny Connect in order to contact the contributor
Submitted on : Thursday, November 24, 2016 - 3:03:58 PM
Last modification on : Wednesday, October 27, 2021 - 3:51:03 PM
Contributor : Marie-Christine Vougny Connect in order to contact the contributor
Submitted on : Thursday, November 24, 2016 - 3:03:58 PM
Last modification on : Wednesday, October 27, 2021 - 3:51:03 PM
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