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Visualizing the Functional Diversification of CD8+ T Cell Responses in Lymph Nodes

Abstract : CD8(+) T cell responses generate effector cells endowed with distinct functional potentials but the contribution of early events in this process is unclear. Here, we have imaged T cells expressing a fluorescent reporter for the activation of the interferon-γ (IFN-γ) locus during priming in lymph nodes. We have demonstrated marked differences in the efficiency of gene activation during stable T cell-dentritic cell (DC) contacts, influenced in part by signal strength. Imaging the first cell division, we have demonstrated that heterogeneity in T cell functional potential was largely apparent as T cells initiated clonal expansion. Moreover, by analyzing the fate of single activated T cells ex vivo, we have provided evidence that these early differences resulted in clonal progenies with distinct functional properties. Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies.
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Submitted on : Thursday, November 24, 2016 - 11:20:29 AM
Last modification on : Monday, January 13, 2020 - 5:08:08 PM
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Hélène Beuneu, Fabrice Lemaître, Jacques Deguine, Hélène D. Moreau, Isabelle Bouvier, et al.. Visualizing the Functional Diversification of CD8+ T Cell Responses in Lymph Nodes. Immunity, Elsevier, 2010, 33 (3), pp.412 - 423. ⟨10.1016/j.immuni.2010.08.016⟩. ⟨pasteur-01402093⟩

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