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Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.

Résumé : Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01402064
Contributor : Marie-Christine Vougny <>
Submitted on : Thursday, November 24, 2016 - 11:01:00 AM
Last modification on : Monday, January 13, 2020 - 5:08:16 PM

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Claire de la Calle, Pierre-Emmanuel Joubert, Helen K W Law, Milena Hasan, Matthew L Albert. Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.. Autophagy, Taylor & Francis, 2011, 7 (9), pp.1045-51. ⟨10.4161/auto.7.9.16252⟩. ⟨pasteur-01402064⟩

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