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Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities.

Abstract : Commensal and pathogenic Escherichia coli adherence to host and environmental surfaces is mediated by a variety of adhesins. Although extensively studied as a model bacterium, 34% of the genes in the E. coli K-12 genome have no known function. We hypothesized that some of them may correspond to functional adhesins. We characterized E. coli K-12 ycb, ybg, yfc, yad, yra, sfm and yeh operons, which display sequence and organizational homologies to type 1 fimbriae exported by the chaperone/usher pathway. We showed that, although these operons are poorly expressed under laboratory conditions, six of them are nevertheless functional when expressed, and promote adhesion to abiotic and/or epithelial cell surfaces. While the studied fimbriae display different binding specificities, we obtained evidence of synergy/interference with other adhesins such as Ag43 or type 1 fimbriae. We showed that their expression is under the negative control of
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01393508
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Charalampia-Georgia Korea, Reana Badouraly, Marie-Christine Prévost, Jean-Marc Ghigo, Christophe Beloin. Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities.. Environmental Microbiology, Wiley-Blackwell, 2010, 12 (7), pp.1957--1977. ⟨10.1111/j.1462-2920.2010.02202.x⟩. ⟨pasteur-01393508⟩

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