Skip to Main content Skip to Navigation
Journal articles

A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections

Abstract : Formation of resilient biofilms on medical devices colonized by pathogenic microorganisms is a major cause of health-care associated infection. While in vitro biofilm analyses led to promising anti-biofilm approaches, little is known about their translation to in vivo situations and on host contribution to the in vivo dynamics of infections on medical devices. Here we have developed an in vivo model of long-term bacterial biofilm infections in a pediatric totally implantable venous access port (TIVAP) surgically placed in adult rats. Using non-invasive and quantitative bioluminescence, we studied TIVAP contamination by clinically relevant pathogens, Escherichia coli , Pseudomonas aeruginosa , Staphylococcus aureus and Staphylococcus epidermidis , and we demonstrated that TIVAP bacterial populations display typical biofilm phenotypes. In our study, we showed that immunocompetent rats were able to control the colonization and clear the bloodstream infection except for up to 30% that suffered systemic infection and death whereas none of the immunosuppressed rats survived the infection. Besides, we mimicked some clinically relevant TIVAP associated complications such as port-pocket infection and hematogenous route of colonization. Finally, by assessing an optimized antibiotic lock therapy, we established that our in vivo model enables to assess innovative therapeutic strategies against bacterial biofilm infections.
Document type :
Journal articles
Complete list of metadata

Cited literature [44 references]  Display  Hide  Download
Contributor : Anne Lassailly-Bondaz Connect in order to contact the contributor
Submitted on : Friday, October 21, 2016 - 4:27:56 PM
Last modification on : Thursday, April 28, 2022 - 2:18:02 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Ashwini Chauhan, David Lebeaux, Benoit Decante, Irene Kriegel, Marie-Christine Escande, et al.. A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections. PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e37281. ⟨10.1371/journal.pone.0037281⟩. ⟨pasteur-01385598⟩



Record views


Files downloads