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Journal articles
Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset
Mickaël J. Ploquin
1
Yoann Madec
2
Armanda Casrouge
3
Nicolas Huot
1
Caroline Passaes
1
Camille Lécuroux
4, 5
Asma Essat
4
Faroudy Boufassa
4
Béatrice Jacquelin
1
Simon P. Jochems
1
Gaël Petitjean
1
Mathieu Angin
1
Kathleen Gärtner
1
Thalía Garcia-Tellez
1
Nicolas Noël
6
Thijs Booiman
7
Brigitte D. Boeser-Nunnink
7
Pierre Roques
6
Asier Saez-Cirion
1
Bruno Vaslin
6
Nathalie Dereudre-Bosquet
6
Françoise Barré-Sinoussi
8
Mathilde Ghislain
4
Christine Rouzioux
9
Olivier Lambotte
6
Matthew L. Albert
3
Cécile Goujard
6
Neeltje Kootstra
7
Laurence Meyer
4
Michaela C. Müller-Trutwin
1, *
*
Corresponding author
Mickaël J. Ploquin 1
Author
Yoann Madec 2
Author IdHAL : yoann-madec ORCID : https://orcid.org/0000-0002-6201-1261
Armanda Casrouge 3
Author
Nicolas Huot 1
Author
Caroline Passaes 1
Author IdHAL : cpassaes ORCID : https://orcid.org/0000-0002-0813-2521
Camille Lécuroux 4, 5
Author
Asma Essat 4
Author
Faroudy Boufassa 4
Author
Béatrice Jacquelin 1
Author IdHAL : beatrice-jacquelin ORCID : https://orcid.org/0000-0002-1896-5092
Simon P. Jochems 1
Author
Gaël Petitjean 1
Author
Mathieu Angin 1
Author
Kathleen Gärtner 1
Author
Thalía Garcia-Tellez 1
Author
Nicolas Noël 6
Author
Thijs Booiman 7
Author
Brigitte D. Boeser-Nunnink 7
Author
Pierre Roques 6
Author
Asier Saez-Cirion 1
Author IdHAL : asier-saez-cirion ORCID : https://orcid.org/0000-0003-2406-7536
Bruno Vaslin 6
Author
Nathalie Dereudre-Bosquet 6
Author
Françoise Barré-Sinoussi 8
Author
Mathilde Ghislain 4
Author
Christine Rouzioux 9
Author
Olivier Lambotte 6
Author
Matthew L. Albert 3
Author
Cécile Goujard 6
Author
Neeltje Kootstra 7
Author
Laurence Meyer 4
Author
Michaela C. Müller-Trutwin 1
Correspondent author IdHAL : michaela-muller-trutwin ORCID : https://orcid.org/0000-0002-3854-2396
1
HIV, Inflammation et persistance (Département Virologie, 25-28 rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
2
Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology (Département Infection et Epidémiologie, 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- PACRI - Pasteur-Cnam risques infectieux et émergents (Paris - France)
- CNAM - Conservatoire National des Arts et Métiers [CNAM] (France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
3
Immunobiologie des Cellules Dendritiques (25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U818 (101, rue de Tolbiac, 75013 Paris - France)
4
CESP - Centre de recherche en épidémiologie et santé des populations (16 avenue Paul Vaillant Couturier 94807 Villejuif Cedex, France - France)
- UVSQ - Université de Versailles Saint-Quentin-en-Yvelines : UMR U1018 (55 avenue de Paris - 78035 Versailles cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1018 (101, rue de Tolbiac, 75013 Paris - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- Hôpital Paul Brousse (12 Avenue Paul Vaillant Couturier, 94800 Villejuif - France)
5
CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay
Centre de Grenoble
Centre de Cadarache
etc - France)
6
IMVA - U1184 - Immunologie des Maladies Virales et Autoimmunes (Site de l'Hôpital de Bicêtre - Faculté de Médecine Paris Sud - 63 rue Gabriel Péri 94276 Le Kremlin-Bicêtre Cedex.
Site du CEA de Fontenay-aux-Roses - 10, route du Panorama - BP 6 - 92265 Fontenay-aux-Roses. - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1184 (101, rue de Tolbiac, 75013 Paris - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/IMETI (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
7
AMC - Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (Meibergdreef 9 1105 AZ Amsterdam - Netherlands)
- UvA - University of Amsterdam [Amsterdam] (Spui 21 1012 WX Amsterdam - Netherlands)
8
Régulation des Infections Rétrovirales (25 rue du Dr Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
9
Laboratoire de Virologie [CHU Necker] (149, rue de Sèvres 75743 PARIS Cedex 15 - France)
- CHU Necker - Enfants Malades [AP-HP] (149 Rue de Sèvres 75015 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
Abstract : Elevated blood CXCL10/IP-10 levels during primary HIV-1 infection (PHI) were described as an independent marker of rapid disease onset, more robust than peak viremia or CD4 cell nadir. IP-10 enhances the recruitment of CXCR3+ cells, which include major HIV-target cells, raising the question if it promotes the establishment of viral reservoirs. We analyzed data from four cohorts of HIV+ patients, allowing us to study IP-10 levels before infection (Amsterdam cohort), as well as during controlled and uncontrolled viremia (ANRS cohorts). We also addressed IP-10 expression levels with regards to lymphoid tissues (LT) and blood viral reservoirs in patients and non-human primates. Pre-existing elevated IP-10 levels but not sCD63 associated with rapid CD4 T-cell loss upon HIV-1 infection. During PHI, IP-10 levels and to a lesser level IL-18 correlated with cell-associated HIV DNA, while 26 other inflammatory soluble markers did not. IP-10 levels tended to differ between HIV controllers with detectable and undetectable viremia. IP-10 was increased in SIV-exposed aviremic macaques with detectable SIV DNA in tissues. IP-10 mRNA was produced at higher levels in the small intestine than in colon or rectum. Jejunal IP-10+ cells corresponded to numerous small and round CD68neg cells as well as to macrophages. Blood IP-10 response negatively correlated with RORC (Th17 marker) gene expression in the small intestine. CXCR3 expression was higher on memory CD4+ T cells than any other immune cells. CD4 T cells from chronically infected animals expressed extremely high levels of intra-cellular CXCR3 suggesting internalization after ligand recognition. Elevated systemic IP-10 levels before infection associated with rapid disease progression. Systemic IP-10 during PHI correlated with HIV DNA. IP-10 production was regionalized in the intestine during early SIV infection and CD68+ and CD68neg haematopoietic cells in the small intestine appeared to be the major source of IP-10.
Document type :
Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01380604
Contributor : Marie-Christine Vougny <>
Submitted on : Thursday, October 13, 2016 - 11:31:56 AM
Last modification on : Wednesday, October 14, 2020 - 4:02:17 AM
Contributor : Marie-Christine Vougny <>
Submitted on : Thursday, October 13, 2016 - 11:31:56 AM
Last modification on : Wednesday, October 14, 2020 - 4:02:17 AM
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journal.ppat.1005774.PDF
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Distributed under a Creative Commons Attribution 4.0 International License
Identifiers
- HAL Id : pasteur-01380604, version 1
- DOI : 10.1371/journal.ppat.1005774
- PUBMED : 27509048
- PUBMEDCENTRAL : PMC4980058
Citation
Mickaël J. Ploquin, Yoann Madec, Armanda Casrouge, Nicolas Huot, Caroline Passaes, et al.. Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset. PLoS Pathogens, Public Library of Science, 2016, 12 (8), pp.11-7. ⟨10.1371/journal.ppat.1005774⟩. ⟨pasteur-01380604⟩
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