-. Using-isol and A. , Prime) according to manufacturer's recommendations. cDNA synthesis was performed on 1 mg of DNase-I (Invitrogen) treated total RNA using an oligodT primer and TaqMan reverse transcriptase (Roche). PCR was performed using the following primers: F-AaeNanos

R. 1. Campbell, C. Keene, K. Brackney, D. Olson, K. Blair et al., Aedes aegypti uses RNA interference in defense against Sindbis virus infection, BMC Microbiology, vol.8, issue.1, p.47, 2008.
DOI : 10.1186/1471-2180-8-47

D. Galiana-arnoux, C. Dostert, A. Schneemann, J. Hoffmann, and J. Imler, Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila, Nature Immunology, vol.69, issue.6, pp.590-597, 2006.
DOI : 10.1038/ni1335

K. Keene, B. Foy, I. Sanchez-vargas, B. Beaty, and C. Blair, RNA interference acts as a natural antiviral response to O'nyong-nyong virus (Alphavirus; Togaviridae) infection of Anopheles gambiae, Proceedings of the National Academy of Sciences, vol.101, issue.49, pp.17240-17245, 2004.
DOI : 10.1073/pnas.0406983101

K. Myles, M. Wiley, E. Morazzani, and Z. Adelman, Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes, Proceedings of the National Academy of Sciences, vol.105, issue.50, pp.19938-19943, 2008.
DOI : 10.1073/pnas.0803408105

I. Sanchez-vargas, J. Scott, B. Poole-smith, A. Franz, and V. Barbosa-solomieu, Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito's RNA Interference Pathway, PLoS Pathogens, vol.35, issue.2, p.1000299, 2009.
DOI : 10.1371/journal.ppat.1000299.t001

R. Van-rij, M. Saleh, B. Berry, C. Foo, and A. Houk, The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster, Genes & Development, vol.20, issue.21, pp.2985-2995, 2006.
DOI : 10.1101/gad.1482006

X. Wang, R. Aliyari, W. Li, H. Li, and K. Kim, RNA Interference Directs Innate Immunity Against Viruses in Adult Drosophila, Science, vol.312, issue.5772, pp.452-454, 2006.
DOI : 10.1126/science.1125694

R. Zambon, V. Vakharia, and L. Wu, RNAi is an antiviral immune response against a dsRNA virus in Drosophila melanogaster, Cellular Microbiology, vol.7, issue.5, pp.880-889, 2006.
DOI : 10.1016/S0092-8674(00)80620-0

M. Siomi, K. Sato, D. Pezic, and A. Aravin, PIWI-interacting small RNAs: the vanguard of genome defence, Nature Reviews Molecular Cell Biology, vol.103, issue.4, pp.246-258, 2011.
DOI : 10.1038/nrm3089

Z. Yan, H. Hu, X. Jiang, V. Maierhofer, and E. Neb, Widespread expression of piRNA-like molecules in somatic tissues, Nucleic Acids Research, vol.39, issue.15, pp.6596-6607, 2011.
DOI : 10.1093/nar/gkr298

J. Brennecke, A. Aravin, A. Stark, M. Dus, and M. Kellis, Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila, Cell, vol.128, issue.6, pp.1089-1103, 2007.
DOI : 10.1016/j.cell.2007.01.043

L. Gunawardane, K. Saito, K. Nishida, K. Miyoshi, and Y. Kawamura, A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila, Science, vol.315, issue.5818, pp.1587-1590, 2007.
DOI : 10.1126/science.1140494

Q. Wu, Y. Luo, R. Lu, N. Lau, and E. Lai, Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs, Proceedings of the National Academy of Sciences, vol.107, issue.4, pp.1606-1611, 2010.
DOI : 10.1073/pnas.0911353107

N. Lau, N. Robine, R. Martin, W. Chung, and Y. Niki, Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line, Genome Research, vol.19, issue.10, pp.1776-1785, 2009.
DOI : 10.1101/gr.094896.109

D. Brackney, J. Scott, F. Sagawa, J. Woodward, and N. Miller, C6/36 Aedes albopictus Cells Have a Dysfunctional Antiviral RNA Interference Response, PLoS Neglected Tropical Diseases, vol.36, issue.7, p.856, 2010.
DOI : 10.1371/journal.pntd.0000856.t001

A. Hess, A. Prasad, A. Ptitsyn, G. Ebel, and K. Olson, Small RNA profiling of Dengue virus-mosquito interactions implicates the PIWI RNA pathway in anti-viral defense, BMC Microbiology, vol.11, issue.1, p.45, 2011.
DOI : 10.1038/nprot.2006.62

J. Scott, D. Brackney, C. Campbell, V. Bondu-hawkins, and B. Hjelle, Comparison of Dengue Virus Type 2-Specific Small RNAs from RNA Interference-Competent and ???Incompetent Mosquito Cells, PLoS Neglected Tropical Diseases, vol.6, issue.10, p.848, 2010.
DOI : 10.1371/journal.pntd.0000848.t002

A. Igarashi, Isolation of a Singh's Aedes albopictus Cell Clone Sensitive to Dengue and Chikungunya Viruses, Journal of General Virology, vol.40, issue.3, pp.531-544, 1978.
DOI : 10.1099/0022-1317-40-3-531

L. Condreay and D. Brown, Exclusion of superinfecting homologous virus by Sindbis virus-infected Aedes albopictus (mosquito) cells, J Virol, vol.58, pp.81-86, 1986.

K. Singh, Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.), Curr Sci, vol.36, pp.56-508, 1967.

S. Kawaoka, N. Izumi, S. Katsuma, and Y. Tomari, 3??? End Formation of PIWI-Interacting RNAs In??Vitro, Molecular Cell, vol.43, issue.6, pp.1015-1022, 2011.
DOI : 10.1016/j.molcel.2011.07.029

M. Horwich, C. Li, C. Matranga, V. Vagin, and G. Farley, The Drosophila RNA Methyltransferase, DmHen1, Modifies Germline piRNAs and Single-Stranded siRNAs in RISC, Current Biology, vol.17, issue.14, pp.1265-1272, 2007.
DOI : 10.1016/j.cub.2007.06.030

M. Borucki, B. Kempf, B. Blitvich, C. Blair, and B. Beaty, La Crosse virus: replication in vertebrate and invertebrate hosts, Microbes and Infection, vol.4, issue.3, pp.341-350, 2002.
DOI : 10.1016/S1286-4579(02)01547-2

C. Rossier, R. Raju, and D. Kolakofsky, LaCrosse virus gene expression in mammalian and mosquito cells, Virology, vol.165, issue.2, pp.539-548, 1988.
DOI : 10.1016/0042-6822(88)90598-3

J. Peleg, Growth of arboviruses in monolayers from subcultured mosquito embryo cells, Virology, vol.35, issue.4, p.617, 1968.
DOI : 10.1016/0042-6822(68)90293-6

R. Siu, R. Fragkoudis, P. Simmonds, C. Donald, and M. Chase-topping, Antiviral RNA Interference Responses Induced by Semliki Forest Virus Infection of Mosquito Cells: Characterization, Origin, and Frequency-Dependent Functions of Virus-Derived Small Interfering RNAs, Journal of Virology, vol.85, issue.6, pp.2907-2917, 2011.
DOI : 10.1128/JVI.02052-10

C. Campbell, W. Black, A. Hess, and B. Foy, Comparative genomics of small RNA regulatory pathway components in vector mosquitoes, BMC Genomics, vol.9, issue.1, 2008.
DOI : 10.1186/1471-2164-9-425

V. Nene, J. Wortman, D. Lawson, B. Haas, and C. Kodira, Genome Sequence of Aedes aegypti, a Major Arbovirus Vector, Science, vol.316, issue.5832, pp.1718-1723, 2007.
DOI : 10.1126/science.1138878

URL : https://hal.archives-ouvertes.fr/hal-00156214

K. Hanley and S. Weaver, Arbovirus Evolution Origin and evolution of viruses, pp.351-392, 2008.

S. Kawaoka, N. Hayashi, Y. Suzuki, H. Abe, and S. Sugano, The Bombyx ovary-derived cell line endogenously expresses PIWI/PIWI-interacting RNA complexes, RNA, vol.15, issue.7, pp.1258-1264, 2009.
DOI : 10.1261/rna.1452209

M. Saleh, M. Tassetto, R. Van-rij, B. Goic, and V. Gausson, Antiviral immunity in Drosophila requires systemic RNA interference spread, Nature, vol.31, issue.7236, pp.346-350, 2009.
DOI : 10.1038/nature07712

URL : https://hal.archives-ouvertes.fr/pasteur-00460853

K. Van-cleef, J. Van-mierlo, M. Van-den-beek, and R. Van-rij, Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture, Methods Mol Biol, vol.721, pp.201-213, 2011.
DOI : 10.1007/978-1-61779-037-9_12

B. Haley, G. Tang, and P. Zamore, In vitro analysis of RNA interference in Drosophila melanogaster, Methods, vol.30, issue.4, pp.330-336, 2003.
DOI : 10.1016/S1046-2023(03)00052-5

V. Gausson and M. Saleh, Viral Small RNA Cloning and Sequencing, Methods Mol Biol, vol.721, pp.107-122, 2011.
DOI : 10.1007/978-1-61779-037-9_6

N. Vodovar, B. Goic, H. Blanc, and M. Saleh, In silico reconstruction of viral genomes from small RNAs improves viral-derived siRNA profiling, J Virol, 2011.

G. Crooks, G. Hon, J. Chandonia, and S. Brenner, WebLogo: A Sequence Logo Generator, Genome Research, vol.14, issue.6, pp.1188-1190, 2004.
DOI : 10.1101/gr.849004