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Nef Is Required for Efficient HIV-1 Replication in Cocultures of Dendritic Cells and Lymphocytes

Abstract : Dendritic cells (DCs) are thought to play a crucial role in the pathogenesis of HIV-1 infection. DCs are believed to transport virus particles to lymph nodes before transfer to CD4(+) lymphocytes. We have investigated the role of Nef in these processes. HIV-1 replication was examined in human immature DC-lymphocyte cocultures and in DCs or lymphocytes separately. Using various R5-tropic and X4-tropic HIV-1 strains and their nef-deleted (Deltanef) counterparts, we show that Nef is required for optimal viral replication in immature DC-T cells clusters and in T lymphocytes. Nef exerts only a marginal role on viral replication in immature DCs alone as well as on virion capture by DCs, long-term intracellular accumulation and transmission of X4 strains to lymphocytes. We also show that wild-type and Deltanef virions are similarly processed for MHC-I restricted exogenous presentation by DCs. Taken together, these results help explain how HIV-1 Nef may affect viral spread and immune responses in the infected host.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01372726
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Submitted on : Tuesday, September 27, 2016 - 3:47:57 PM
Last modification on : Monday, January 13, 2020 - 5:08:17 PM

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Caroline Petit, Florence Buseyne, Claire Boccaccio, Jean-Pierre Abastado, Jean-Michel Heard, et al.. Nef Is Required for Efficient HIV-1 Replication in Cocultures of Dendritic Cells and Lymphocytes. Virology, Elsevier, 2001, 286 (1), pp.225 - 236. ⟨10.1006/viro.2001.0984⟩. ⟨pasteur-01372726⟩

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