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DC-SIGN promotes exogenous MHC-I-restricted HIV-1 antigen presentation.

Abstract : Dendritic cells (DCs) facilitate HIV-1 spread in the host by capturing virions and transferring them to permissive lymphocytes in lymphoid organs. Lectins such as DC-specific ICAM-grabbing non-integrin (DC-SIGN) are involved in HIV-1 uptake by DCs, through high-affinity binding to viral envelope glycoproteins. We examined the role of DC-SIGN on the fate of incoming virions and on major histocompatibility complex class I (MHC-I)-restricted HIV-1 antigen presentation. We show that DC-SIGN expression in B-cell lines dramatically enhances viral internalization. In these cells, and also in primary DCs, most of the captured virions are rapidly degraded, likely in a lysosomal compartment. In addition, a fraction of incoming viral material is processed by the proteasome, leading to activation of anti-HIV-specific cytotoxic T lymphocytes (CTLs) by DC-SIGN-expressing cells. In DCs, DC-SIGN is not the only receptor involved, and redundant pathways of virus capture leading to antigen presentation likely coexist. Altogether, our results highlight new aspects of DC-SIGN interactions with HIV-1. The lectin does not significantly protect captured virions against degradation and promotes MHC-I exogenous presentation of HIV-1 antigens.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01372698
Contributor : Isma Ziani <>
Submitted on : Tuesday, September 27, 2016 - 3:29:28 PM
Last modification on : Monday, January 13, 2020 - 5:08:07 PM

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Arnaud Moris, Cinzia Nobile, Florence Buseyne, Françoise Porrot, Jean-Pierre Abastado, et al.. DC-SIGN promotes exogenous MHC-I-restricted HIV-1 antigen presentation.. Blood, American Society of Hematology, 2004, 103 (7), pp.2648-54. ⟨10.1182/blood-2003-07-2532⟩. ⟨pasteur-01372698⟩

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