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Journal articles
Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma.
Claire Martel-Jantin
1
Claudia Filippone
1
Olivier Cassar
1
M Peter
2
Gorana Tomasic
3, 4
Philippe Vielh
5
Josette Brière
6
Tony Petrella
7
Marie-Hélène Aubriot-Lorton
8
Laurent Mortier
9
Grégory Jouvion
10
Xavier Sastre-Garau
2
Caroline Robert
11
Antoine Gessain
1, *
*
Corresponding author
Marie-Hélène Aubriot-Lorton 8
Author
Laurent Mortier 9
Author IdHAL : laurent-mortier
Grégory Jouvion 10
Author
Xavier Sastre-Garau 2
Author
Caroline Robert 11
Author
Antoine Gessain 1
Correspondent author
1
EPVO (UMR_3569 / U-Pasteur_3) - Epidémiologie et Physiopathologie des Virus Oncogènes (Département de Virologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR_3569 (France)
- UPD7 - Université Paris Diderot - Paris 7 : UMR_3569 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- Institut Pasteur [Paris] : U-Pasteur_3 (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
2
Institut Curie [Paris] ( 26 rue d'Ulm - 75248 Paris - France)
3
Département de biologie et pathologie médicales [Gustave Roussy] (Gustave Roussy. 114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
- IGR - Institut Gustave Roussy (114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
4
IGR - Institut Gustave Roussy (114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
5
Laboratoire de recherche translationnelle (Gustave Roussy. 114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
- Direction de la recherche [Gustave Roussy] (Gustave Roussy. 114, rue Édouard-Vaillant 94805 Villejuif Cedex - France)
- IGR - Institut Gustave Roussy (114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
6
Service d'anatomo-pathologie [Paris] (1 avenue Claude Vellefaux 75475 Paris cedex 10 - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris] (200 rue du Faubourg Saint-Denis 75475 Paris (Nord) Cedex 10 - France)
7
Centre de pathologie (33 Rue Nicolas Bornier, 21000 Dijon - France)
8
Service d'anatomie et de cytologie pathologiques (France)
- CHU Dijon - Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon - 14 rue Paul Gaffarel - 21079 Dijon - France)
9
Service de dermatologie (Hôpital Claude Huriez, 1 Place de Verdun 59037Lille Cedex - France)
- CHRU Lille - Centre Hospitalier Régional Universitaire [Lille] (2, avenue Oscar Lambret - 59037 Lille Cedex - France)
10
Histopathologie humaine et Modèles animaux (Département Infection et Epidémiologie, 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
11
Service de dermatologie (Villejuif - France)
- Département de médecine oncologique [Gustave Roussy] (Gustave Roussy. 114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
- IGR - Institut Gustave Roussy (114, rue Édouard-Vaillant 94805 Villejuif Cedex -France - France)
Abstract : Merkel cell polyomavirus (MCPyV) is associated to Merkel cell carcinoma (MCC). We studied 113 MCC tumoral skin lesions originating from 97 patients. MCPyV detection was higher in fresh-frozen (FF) biopsies (94%) than in formalin-fixed paraffin-embedded biopsies (39-47%). Mean viral load in FF tumor was of 7.5 copies per cell with a very wide range (0.01-95.4). Nineteen complete sequences of LTAg were obtained, mainly from FF biopsies when the viral load was high. Seventeen showed stop codons, all localized downstream of the pRb protein binding domain. Sequence comparison and phylogenetic analysis showed that all sequences clustered in the large C clade of MCPyV strains. MCPyV integration was demonstrated in 19 out of 27 FF MCC DNA biopsies without evidence of specific host cellular genome integration site. In 13/19 cases, the viral junction was located within the second exon of the LTAg, after the pRB binding domain.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01370953
Contributor : Isma Ziani <>
Submitted on : Monday, December 26, 2016 - 4:07:10 PM
Last modification on : Wednesday, August 19, 2020 - 11:17:19 AM
Contributor : Isma Ziani <>
Submitted on : Monday, December 26, 2016 - 4:07:10 PM
Last modification on : Wednesday, August 19, 2020 - 11:17:19 AM
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- HAL Id : pasteur-01370953, version 1
- DOI : 10.1016/j.virol.2012.01.018
- PUBMED : 22342276
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PASTEUR | CNRS | UNIV-PARIS7 | CURIE | FNCLCC | USPC | IGR | UNIV-PARIS | UP-SCIENCES | PSL
Citation
Claire Martel-Jantin, Claudia Filippone, Olivier Cassar, M Peter, Gorana Tomasic, et al.. Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma.. Virology, Elsevier, 2012, 426 (2), pp.134-42. ⟨10.1016/j.virol.2012.01.018⟩. ⟨pasteur-01370953⟩
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