HTLV-1-associated inflammatory myopathies: low proviral load and moderate inflammation in 13 patients from West Indies and West Africa.

Marion Desdouits 1, 2 Olivier Cassar 2 Thierry Maisonobe 3, 4 Alexandra Desrames 2 Achille Aouba 5 Olivier Hermine 6 Jacqueline Mikol 7 Marc Polivka 7 Isabelle Penisson-Besnier 8 Pascale Marcorelles 9 Thomas Papo 10 Fabien Zagnoli 11 Thomas Papo 12 Arnaud Lacour 13 Zahir Amoura 14 Julien Haroche 15, 16 Patrick Cherin 17 Antonio Teixeira 4 Olivier Benveniste 14 Serge Herson 18 Anne-Sophie Morin 19 Franck Mortreux 20 Eric Wattel 20 Michel Huerre Marie-Christine Cumont 2 Sandra Martin-Latil 2 Gillian Butler-Browne 18 Olivier Gout 21 Graham Taylor Antoine Gessain 2 Simona Ozden 2 Pierre-Emmanuel Ceccaldi 1, 2, *
Abstract : The Human T-cell Leukemia Virus type 1 (HTLV-1) is the causative agent of several inflammatory diseases, including HTLV-1-associated inflammatory myopathies (HAIM). Little is known about the virological and immunological characteristics of this viral disease. To characterize the histological and virological features of HAIM patients, in order to better understand the pathogenetic mechanisms and unravel new biological markers of this disease. We conducted a retrospective study on 13 patients with HAIM, based on blood and muscle samples. We included blood samples from HTLV-1-infected individuals without myopathy as controls. Muscle biopsies were used for a broad immunohistological evaluation of tissue damage and inflammation, as well as identification of infected cells through in situ hybridization. DNA extracted from patients' PBMC was used to identify the virus genotype by sequencing and to assess the proviral load by quantitative PCR. Anti-viral antibodies in plasma samples were titrated by indirect immunofluorescence. Patients originate from HTLV-1 endemic areas, the West Indies and West Africa. Histological alterations and inflammation in patients muscles were mostly moderate, with classical features of idiopathic myositis and rare HTLV-1-infected infiltrating cells. In all patients, HTLV-1 belonged to the A subtype, transcontinental subgroup. Anti-HTLV-1 antibodies titers were high, but the proviral load was not elevated compared to asymptomatic HTLV-1 carriers. We show here that muscle inflammation is moderate in HAIM, and accompanied by a low HTLV-1 proviral load, suggesting that the pathogenetic events do not exactly mirror those of other HTLV-1-associated inflammatory diseases.
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Submitted on : Friday, September 23, 2016 - 3:13:42 PM
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Marion Desdouits, Olivier Cassar, Thierry Maisonobe, Alexandra Desrames, Achille Aouba, et al.. HTLV-1-associated inflammatory myopathies: low proviral load and moderate inflammation in 13 patients from West Indies and West Africa.. Journal of Clinical Virology, Elsevier, 2013, 57 (1), pp.70-6. ⟨10.1016/j.jcv.2012.12.016⟩. ⟨pasteur-01370950⟩

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