Multidisciplinary analysis of a nontoxigenic Clostridium difficile strain with stable resistance to metronidazole.

Ines Moura (1) , Marc Monot (2) , Chiara Tani (3) , Patrizia Spigaglia (1) , Fabrizio Barbanti (1) , Nathalie Norais (3) , Bruno Dupuy (2) , Emilio Bouza (4) , Paola Mastrantonio (1)

1 Departmet of Infectious, Parasitic and Immune-Mediated Disease
2 PBA (U-Pasteur_6) - Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes
3 Structural Mass Spectrometry and Proteomics Unit [Italy]
4 Hospital General Universitario "Gregorio Marañón" [Madrid]

Stable resistance to metronidazole in a nontoxigenic Clostridium difficile strain was investigated at both the genomic and proteomic levels. Alterations in the metabolic pathway involving the pyruvate-ferredoxin oxidoreductase were found, suggesting that reduction of metronidazole, required for its activity, may be less efficient in this strain. Proteomic studies also showed a cellular response to oxidative stress.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology

Ines Moura, Marc Monot, Chiara Tani, Patrizia Spigaglia, Fabrizio Barbanti, et al.. Multidisciplinary analysis of a nontoxigenic Clostridium difficile strain with stable resistance to metronidazole.. Antimicrobial Agents and Chemotherapy, 2014, 58 (8), pp.4957-60. ⟨10.1128/AAC.02350-14⟩. ⟨pasteur-01370760⟩