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Repressor activity of the RpoS/σS-dependent RNA polymerase requires DNA binding

Abstract : The RpoS/σS sigma subunit of RNA polymerase (RNAP) activates transcription of stationary phase genes in many Gram-negative bacteria and controls adaptive functions, including stress resistance, biofilm formation and virulence. In this study, we address an important but poorly understood aspect of σS-dependent control, that of a repressor. Negative regulation by σ S has been proposed to result largely from competition between σS and other σ factors for binding to a limited amount of core RNAP (E). To assess whether σS binding to E alone results in significant downregulation of gene expression by other σfactors, we characterized an rpoS mutant of Salmonella enterica serovar Typhimurium producing a σS protein proficient for EσS complex formation but deficient in promoter DNA binding. Genome expression profiling and physiological assays revealed that this mutant was defective for negative regulation , indicating that gene repression by σS requires its binding to DNA. Although the mechanisms of repression by σS are likely specific to individual genes and environmental conditions, the study of transcription downregulation of the succinate dehydrogenase operon suggests that σcompetition at the promoter DNA level plays an important role in gene repression by EσS .
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Nucl. Acids Res.-2015-Lévi-Me...
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Corinne Levi-Meyrueis, Véronique Monteil, Odile Sismeiro, Marie-Agnès Dillies, Annie Kolb, et al.. Repressor activity of the RpoS/σS-dependent RNA polymerase requires DNA binding. Nucleic Acids Research, Oxford University Press, 2015, 43 (3), pp.1456 - 1468. ⟨10.1093/nar/gku1379⟩. ⟨pasteur-01370752⟩

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