Skip to Main content Skip to Navigation
Journal articles

The Rift Valley fever accessory proteins NSm and P78/NSm-GN are distinct determinants of virus propagation in vertebrate and invertebrate hosts.: Role of NSm-related proteins in RVFV infection

Abstract : Rift Valley fever virus (RVFV) is an enzootic virus circulating in Africa that is transmitted to its vertebrate host by a mosquito vector and causes severe clinical manifestations in humans and ruminants. RVFV has a tripartite genome of negative or ambisense polarity. The M segment contains five in-frame AUG codons that are alternatively used for the synthesis of two major structural glycoproteins, GN and GC, and at least two accessory proteins, NSm, a 14-kDa cytosolic protein, and P78/NSm-GN, a 78-kDa glycoprotein. To determine the relative contribution of P78 and NSm to RVFV infectivity, AUG codons were knocked out to generate mutant viruses expressing various sets of the M-encoded proteins. We found that, in the absence of the second AUG codon used to express NSm, a 13-kDa protein corresponding to an N-terminally truncated form of NSm, named NSm', was synthesized from AUG 3. None of the individual accessory proteins had any significant impact on RVFV virulence in mice. However, a mutant virus lacking both NSm and NSm' was strongly attenuated in mice and grew to reduced titers in murine macrophages, a major target cell type of RVFV. In contrast, P78 was not associated with reduced viral virulence in mice, yet it appeared as a major determinant of virus dissemination in mosquitoes. This study demonstrates how related accessory proteins differentially contribute to RVFV propagation in mammalian and arthropod hosts.
Complete list of metadatas

Cited literature [54 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01325890
Contributor : Jean-Jacques Panthier <>
Submitted on : Thursday, June 2, 2016 - 5:59:31 PM
Last modification on : Saturday, June 6, 2020 - 4:14:02 PM
Long-term archiving on: : Saturday, September 3, 2016 - 11:27:28 AM

File

Kreher Emerg Microbes Inf 2014...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Felix Kreher, Carole Tamietti, Céline Gommet, Laurent Guillemot, Myriam Ermonval, et al.. The Rift Valley fever accessory proteins NSm and P78/NSm-GN are distinct determinants of virus propagation in vertebrate and invertebrate hosts.: Role of NSm-related proteins in RVFV infection. Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2014, 3 (10), pp.e71. ⟨10.1038/emi.2014.71⟩. ⟨pasteur-01325890⟩

Share

Metrics

Record views

551

Files downloads

607