Skip to Main content Skip to Navigation
Journal articles

Adult Neurogenesis Restores Dopaminergic Neuronal Loss in the Olfactory Bulb: Dopaminergic cell regeneration

Abstract : Subventricular zone (SVZ) neurogenesis continuously provides new GABA-and dopamine (DA)-containing interneurons for the olfac-tory bulb (OB) in most adult mammals. DAergic interneurons are located in the glomerular layer (GL) where they participate in the processing of sensory inputs. To examine whether adult neurogenesis might contribute to regeneration after circuit injury in mice, we induce DAergic neuronal loss by injecting 6-hydroxydopamine (6-OHDA) in the dorsal GL or in the right substantia nigra pars compacta. We found that a 6-OHDA treatment of the OB produces olfactory deficits and local inflammation and partially decreases the number of neurons expressing the enzyme tyrosine hydroxylase (TH) near the injected site. Blockade of inflammation by minocycline treatment immediately after the 6-OHDA administration rescued neither TH ϩ interneuron number nor the olfactory deficits, suggesting that the olfactory impairments are most likely linked to TH ϩ cell death and not to microglial activation. TH ϩ interneuron number was restored 1 month later. This rescue resulted at least in part from enhanced recruitment of immature neurons targeting the lesioned GL area. Seven days after 6-OHDA lesion in the OB, we found that the integration of lentivirus-labeled adult-born neurons was biased: newly formed neurons were preferentially incorporated into glomerular circuits of the lesioned area. Behavioral rehabilitation occurs 2 months after lesion. This study establishes a new model into which loss of DAergic cells could be compensated by recruiting newly formed neurons. We propose that adult neurogenesis not only replenishes the population of DAergic bulbar neurons but that it also restores olfactory sensory processing.
Document type :
Journal articles
Complete list of metadatas

Cited literature [18 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01300275
Contributor : Françoise Lazarini <>
Submitted on : Saturday, April 9, 2016 - 6:01:24 PM
Last modification on : Monday, January 13, 2020 - 5:08:10 PM
Long-term archiving on: : Tuesday, November 15, 2016 - 12:16:09 AM

File

14430.full.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Françoise Lazarini, Marie-Madeleine Gabellec, Carine Moigneu, Fabrice de Chaumont, Jean-Christophe Olivo-Marin, et al.. Adult Neurogenesis Restores Dopaminergic Neuronal Loss in the Olfactory Bulb: Dopaminergic cell regeneration. Journal of Neuroscience, Society for Neuroscience, 2014, 34 (43), pp.14430-42. ⟨10.1523/JNEUROSCI.5366-13.2014⟩. ⟨pasteur-01300275⟩

Share

Metrics

Record views

337

Files downloads

447