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Activation of the NLRP3 Inflammasome by Group B Streptococci

Abstract : Group B Streptococcus (GBS) is a frequent agent of life-threatening sepsis and meningitis in neonates and adults with predisposing conditions. We tested the hypothesis that activation of the inflammasome, an inflammatory signaling complex, is involved in host defenses against this pathogen. We show in this study that murine bone marrow-derived conventional dendritic cells responded to GBS by secreting IL-1β and IL-18. IL-1β release required both pro–IL-1β transcription and caspase-1–dependent proteolytic cleavage of intracellular pro–IL-1β. Dendritic cells lacking the TLR adaptor MyD88, but not those lacking TLR2, were unable to produce pro–IL-1β mRNA in response to GBS. Pro–IL-1β cleavage and secretion of the mature IL-1β form depended on the NOD-like receptor family, pyrin domain containing 3 (NLRP3) sensor and the apoptosis-associated speck-like protein containing a caspase activation and recruitment domain adaptor. Moreover, activation of the NLRP3 inflammasome required GBS expression of β-hemolysin, an important virulence factor. We further found that mice lacking NLRP3, apoptosis-associated speck-like protein, or caspase-1 were considerably more susceptible to infection than wild-type mice. Our data link the production of a major virulence factor by GBS with the activation of a highly effective anti-GBS response triggered by the NLRP3 inflammasome.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01300173
Contributor : Anne Lassailly-Bondaz <>
Submitted on : Friday, April 8, 2016 - 5:53:09 PM
Last modification on : Tuesday, June 16, 2020 - 2:22:01 PM

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Alessandro Costa, Rahul Gupta, Giacomo Signorino, Antonio Malara, Francesco Cardile, et al.. Activation of the NLRP3 Inflammasome by Group B Streptococci. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2012, 188 (4), pp.1953-1960. ⟨10.4049/jimmunol.1102543⟩. ⟨pasteur-01300173⟩

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