Rouxiella chamberiensis gen. nov., sp. nov., a new Enterobacteriaceae isolated from parenteral nutrition bags.

Anne Le Fleche-Mateos (1, 2) , Marion Levast (3) , Fabienne Lomprez (2, 1) , Yolande Arnoux (2, 1) , Clément Andonian (2, 1) , Michel Perraud (4) , Véronique Vincent (2, 1) , Meriadeg Ar Gouilh (1, 2) , Jean-Michel Thiberge (2, 1) , Mathias Vandenbogaert (1, 2) , Laure Diancourt (2, 1) , Valérie Caro (2, 1) , Ana Maria Burguiere (1, 2) , Jean-Claude Manuguerra (2, 1)

1 ERI - Environnement et Risques infectieux - Environment and Infectious Risks
2 CIBU - Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
3 Laboratoire de Bactériologie
4 Laboratoire de Biologie-Sécurité-Environnement

Parenteral nutrition bags for newborns were found contaminated by a previously undescribed 23 Enterobacteriaceae. The six isolates studied by rrs - (encoding 16S rRNA) and multilocus 24 sequence analysis (MLSA) formed a discrete branch close to genera Ewingella, Rahnella, 25 Yersinia, Hafnia and Serratia. Phenotypically, the new taxon was distinct from these four 26 genera. The new taxon gave positive Voges-Proskauer, Simmons citrate, o-nitrophenyl-β-27 galactoside hydrolysis tests; fermented D-glucose, D-mannitol, L-rhamnose, D-melibiose, L-28 arabinose, D-xylose, and hydrolyzed esculin; did not ferment maltose, trehalose, raffinose, D-29 sorbitol, sucrose and D-cellobiose. The following tests, motility, gas production, urease, 30 gelatinase, and nitrate reduction were also negative. All isolates failed to grow at 37°C. 31 Therefore, the new taxon is proposed as a new species and genus and named Rouxiella 32 chamberiensis gen. nov., sp. nov. The type strain is 130333T (= CIP 110714T = DSM 28324T). 33 The G+C content of the type strain DNA was 53 mol%.

Domains: Life Sciences [q-bio]; Microbiology and Parasitology

Anne Le Fleche-Mateos, Marion Levast, Fabienne Lomprez, Yolande Arnoux, Clément Andonian, et al.. Rouxiella chamberiensis gen. nov., sp. nov., a new Enterobacteriaceae isolated from parenteral nutrition bags.. International Journal of Systematic and Evolutionary Microbiology, 2015, ⟨10.1099/ijs.0.000179⟩. ⟨pasteur-01134699⟩