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Riboswitches. Sequestration of a two-component response regulator by a riboswitch-regulated noncoding RNA.

Abstract : Riboswitches are ligand-binding elements contained within the 5' untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. Here, we show that in Listeria monocytogenes, a riboswitch that binds vitamin B12 controls expression of a noncoding regulatory RNA, Rli55. Rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require B12 as a cofactor. Defects in ethanolamine utilization, or in its regulation by Rli55, significantly attenuate Listeria virulence in mice. Rli55 functions by sequestering the two-component response regulator EutV by means of a EutV-binding site contained within the RNA. Thus, Rli55 is a riboswitch-regulated member of the small group of regulatory RNAs that function by sequestering a protein and reveals a distinctive mechanism of signal integration in bacterial gene regulation.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01120664
Contributor : Marie-Therese Vicente <>
Submitted on : Friday, April 24, 2015 - 9:29:19 AM
Last modification on : Thursday, October 15, 2020 - 2:42:03 PM
Long-term archiving on: : Wednesday, April 19, 2017 - 4:44:21 AM

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J R Mellin, Mikael Koutero, Daniel Dar, Marie-Anne Nahori, Rotem Sorek, et al.. Riboswitches. Sequestration of a two-component response regulator by a riboswitch-regulated noncoding RNA.. Science, American Association for the Advancement of Science, 2014, 345 (6199), pp.940-3. ⟨10.1126/science.1255083⟩. ⟨pasteur-01120664⟩

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