Skip to Main content Skip to Navigation
Journal articles

Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups.

Abstract : Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely nonoverlapping, isolates with combined virulence and resistance features were detected.
Document type :
Journal articles
Complete list of metadatas

Cited literature [39 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01118613
Contributor : Marie Lemesle <>
Submitted on : Thursday, February 19, 2015 - 2:38:28 PM
Last modification on : Monday, October 19, 2020 - 11:12:37 AM
Long-term archiving on: : Sunday, April 16, 2017 - 10:04:48 AM

File

14-0206.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Citation

Suzanne Bialek-Davenet, Alexis Criscuolo, Florent Ailloud, Virginie Passet, Louis Jones, et al.. Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups.. Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2014, 20 (11), pp.1812-20. ⟨10.3201/eid2011.140206⟩. ⟨pasteur-01118613⟩

Share

Metrics

Record views

1520

Files downloads

1608