Characterization of Extended-Spectrum Beta-Lactamase–Producing Salmonella enterica Serotype Brunei and Heidelberg at the Hussein Dey Hospital in Algiers (Algeria)

Rachida Kermas (1) , Abdelaziz Touati (1) , Lucien Brasme (2) , Elisabeth Le Magrex-Debar (2) , Sadjia Mehrane (3) , François-Xavier Weill (4) , Christophe de Champs (2)

1 UB - Université Abderrahmane Mira [Université de Béjaïa] = University Abderrahmane Mira [University of Béjaïa]
2 Hôpital Robert Debré
3 CHU Nafissa Hamoud [Hussein Dey]
4 CNR-ESS - Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella

The purpose of this work was to study the genetic determinants responsible for extended-spectrum cephalosporin (ESC) resistance of Salmonella collected during the period of 1995-2008 at the Hussein Dey hospital in Algiers (Algeria). Fourteen ESC-resistant Salmonella isolates were tested towards 22 antimicrobial agents. Polymerase chain reaction (PCR) and sequencing were used to determine the underlying genetic determinants responsible for the extended-spectrum beta-lactamase (ESBL) phenotypes. Enterobacterial Repetitive Intergenic Consensus PCR was employed to type the isolates. All tested isolates were resistant to ticarcillin, ticarcillin-clavulanate, piperacillin, cefuroxime, aztreonam, ceftazidime, cefotaxime (except two isolates), cefepime, and cefpirome. PCR and DNA sequencing identified these ESBLs as TEM-48 (n=6), TEM-4 (n=3), CTX-M-15 (n=4), and one new TEM, designated TEM-188. Thus, continued surveillance for the presence of ESBL-producing (non-typhoidal) salmonellae in Algeria is essential.

Domaines: Santé publique et épidémiologie

Rachida Kermas, Abdelaziz Touati, Lucien Brasme, Elisabeth Le Magrex-Debar, Sadjia Mehrane, et al.. Characterization of Extended-Spectrum Beta-Lactamase–Producing Salmonella enterica Serotype Brunei and Heidelberg at the Hussein Dey Hospital in Algiers (Algeria). 2012. ⟨pasteur-01115981⟩