Diversity of archaeal type IV pilin-like structures. 613 Extremophiles, pp.403-410, 2009. ,
Architecture of the type II secretion and type IV pilus machineries, Future Microbiology, vol.5, issue.8, pp.1203-1218, 2010. ,
DOI : 10.2217/fmb.10.76
Production and ultrastructure of lysozyme and 617 ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli, J Bacteriol, vol.618, issue.93, pp.427-437, 1967. ,
Implementation of the CHARMM Force Field in GROMACS: Analysis of Protein Stability Effects from Correction Maps, Virtual Interaction Sites, and Water Models, Journal of Chemical Theory and Computation, vol.6, issue.2, pp.459-466, 2010. ,
DOI : 10.1021/ct900549r
Detailed structural and assembly 627 model of the type II secretion pilus from sparse data, Proc Natl Acad Sci, vol.107, pp.628-13081, 2010. ,
A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili, Molecular Microbiology, vol.181, issue.6, pp.1510-1522, 2006. ,
DOI : 10.1093/emboj/19.23.6408
The Ins and Outs of DNA Transfer in Bacteria, Science, vol.310, issue.5753, pp.1456-1460, 2005. ,
DOI : 10.1126/science.1114021
Type II secretion: a protein secretion system for all seasons. Trends 635 Microbiol, pp.581-588, 2005. ,
Type IV pili: paradoxes in form and function, Current Opinion in Structural Biology, vol.18, issue.2, pp.267-277, 2008. ,
DOI : 10.1016/j.sbi.2007.12.009
The XcpV/GspI pseudopilin has a central role in the assembly of a 646 quaternary complex within the T2SS pseudopilus, J Biol Chem, vol.284, pp.34580-34589, 2009. ,
XcpX controls 648 biogenesis of the Pseudomonas aeruginosa XcpT-containing pseudopilus, J Biol, vol.649, issue.280, pp.31378-31389, 2005. ,
The type II secretion arrowhead: the structure of GspI???GspJ???GspK, Nature Structural & Molecular Biology, vol.15, issue.5, p.651, 2008. ,
DOI : 10.1016/S0959-440X(00)00129-9
Signal Recognition Particle-Dependent Inner Membrane Targeting of the PulG Pseudopilin Component of a Type II Secretion System, Journal of Bacteriology, vol.189, issue.5, pp.1783-1793, 2007. ,
DOI : 10.1128/JB.01230-06
URL : https://hal.archives-ouvertes.fr/hal-00138935
Towards the Identification of Type II Secretion Signals in a Nonacylated Variant of Pullulanase from Klebsiella oxytoca, Journal of Bacteriology, vol.187, issue.20, pp.7045-657, 2005. ,
DOI : 10.1128/JB.187.20.7045-7055.2005
Pseudomonas aeruginosa Minor Pilins Are Incorporated into Type IV Pili, Journal of Molecular Biology, vol.398, issue.3, pp.444-461, 2010. ,
DOI : 10.1016/j.jmb.2010.03.028
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae, Molecular Microbiology, vol.377, issue.3, pp.786-798, 2011. ,
DOI : 10.1111/j.1365-2958.2010.07487.x
Type IV Pilin Structures: Insights on Shared Architecture, Fiber Assembly, Receptor Binding and Type II Secretion, Journal of Molecular Microbiology and Biotechnology, vol.11, issue.3-5, pp.192-207, 2006. ,
DOI : 10.1159/000094054
GROMACS 4:?? Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation, Journal of Chemical Theory and Computation, vol.4, issue.3, pp.435-447, 2008. ,
DOI : 10.1021/ct700301q
A bacterial two-hybrid system based on a reconstituted signal transduction pathway, Proceedings of the National Academy of Sciences, vol.95, issue.10, pp.5752-671, 1998. ,
DOI : 10.1073/pnas.95.10.5752
Structure of the GspK-GspI-GspJ complex from the 676 enterotoxigenic Escherichia coli type 2 secretion system, Nat Struct Mol Biol, vol.15, pp.677-462, 2008. ,
Characterization of interactions between PilA from 679 Pseudomonas aeruginosa strain K and a model membrane, J Phys Chem B, vol.115, pp.680-8004, 2011. ,
Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives, MGG Molecular & General Genetics, vol.36, issue.2, pp.285-290, 1986. ,
DOI : 10.1007/BF00430440
Yin D and Karplus 688 M (1998) All-atom empirical potential for molecular modeling and dynamics studies 689 of proteins, J Phys Chem B, vol.102, pp.3586-3616 ,
Update of the CHARMM All-Atom 692, 2010. ,
A force-dependent switch reverses type IV pilus retraction, Proceedings of the National Academy of Sciences, vol.101, issue.30, pp.10961-10966, 2004. ,
DOI : 10.1073/pnas.0402305101
Molecular cloning: a laboratory manual. Cold Spring 697 Harbor Laboratory, p.698, 1982. ,
Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold 699 Spring Harbor, p.700, 1972. ,
P. aeruginosa PilT structures with and 701 without nucleotide reveal a dynamic type IV pilus retraction motor, J Mol Biol, vol.400, pp.702-1011, 2010. ,
Type IV pili: e pluribus unum?, Molecular Microbiology, vol.181, issue.4, pp.827-837, 2008. ,
DOI : 10.1172/JCI30727
Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE, Journal of Bacteriology, vol.182, issue.8, pp.2142-2152, 2000. ,
DOI : 10.1128/JB.182.8.2142-2152.2000
Processing and methylation of PulG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca, Molecular Microbiology, vol.90, issue.2, pp.295-308, 1993. ,
DOI : 10.1016/0378-1119(87)90365-9
The complete general secretory pathway in gram-negative bacteria. 713, Microbiol. Rev, vol.57, pp.50-108, 1993. ,
An inner membrane platform in the type II secretion machinery of Gram-negative bacteria, EMBO reports, vol.143, issue.3, pp.244-248, 2001. ,
DOI : 10.1093/embo-reports/kve042
Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli, Cell, vol.46, issue.6, pp.921-928, 0718. ,
DOI : 10.1016/0092-8674(86)90074-7
Type II Secretion and Pathogenesis, Infection and Immunity, vol.69, issue.6, pp.3523-3535, 2001. ,
DOI : 10.1128/IAI.69.6.3523-3535.2001
Pilus formation and protein secretion by the same machinery in Escherichia coli, The EMBO Journal, vol.96, issue.10, pp.2221-2228, 2000. ,
DOI : 10.1093/emboj/19.10.2221
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, vol.166, issue.2, pp.368-379, 1987. ,
DOI : 10.1016/0003-2697(87)90587-2
Type IV-Like Pili Formed by the Type II Secreton: Specificity, Composition, Bundling, Polar Localization, and Surface Presentation of Peptides, Journal of Bacteriology, vol.185, issue.11, pp.3416-3428, 2003. ,
DOI : 10.1128/JB.185.11.3416-3428.2003
A conserved set of pilin-like molecules controls type IV pilus 730 dynamics and organelle-associated functions in Neisseria gonorrhoeae, Mol 731 Microbiol, pp.903-917, 2005. ,
GspK simulations (red) and the six main clusters of the GspJ alone 798 simulation (gray), shown in the same view as (A) (C) Representative structures of GspI, p.799 ,