Skip to Main content Skip to Navigation
Journal articles

SAMHD1 restricts HIV-1 cell-to-cell transmission and limits immune detection in monocyte-derived dendritic cells.

Abstract : SAMHD1 is a viral restriction factor expressed in dendritic cells and other cells, inhibiting infection by cell-free human immunodeficiency virus type 1 (HIV-1) particles. SAMHD1 depletes the intracellular pool of deoxynucleoside triphosphates, thus impairing HIV-1 reverse transcription and productive infection in noncycling cells. The Vpx protein from HIV-2 or simian immunodeficiency virus (SIVsm/SIVmac) antagonizes the effect of SAMHD1 by triggering its degradation. A large part of HIV-1 spread occurs through direct contacts between infected cells and bystander target cells. Here, we asked whether SAMHD1 impairs direct HIV-1 transmission from infected T lymphocytes to monocyte-derived dendritic cells (MDDCs). HIV-1-infected lymphocytes were cocultivated with MDDCs that have been pretreated or not with Vpx or with small interfering RNA against SAMHD1. We show that in the cocultures, SAMHD1 significantly inhibits productive cell-to-cell transmission to target MDDCs and prevents the type I interferon response and expression of the interferon-stimulated gene MxA. Therefore, SAMHD1, by controlling the sensitivity of MDDCs to HIV-1 infection during intercellular contacts, impacts their ability to sense the virus and to trigger an innate immune response.
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01109474
Contributor : Isma Ziani <>
Submitted on : Monday, January 26, 2015 - 1:58:56 PM
Last modification on : Monday, January 13, 2020 - 5:08:10 PM

Links full text

Identifiers

Collections

Citation

Isabel Puigdomènech, Nicoletta Casartelli, Françoise Porrot, Olivier Schwartz. SAMHD1 restricts HIV-1 cell-to-cell transmission and limits immune detection in monocyte-derived dendritic cells.. Journal of Virology, American Society for Microbiology, 2013, 87 (5), pp.2846-56. ⟨10.1128/JVI.02514-12⟩. ⟨pasteur-01109474⟩

Share

Metrics

Record views

177