Skip to Main content Skip to Navigation
New interface
Journal articles

Quality assessment and optimization of purified protein samples: why and how?

Abstract : Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and misleading observations in downstream applications. In this review, we aim at proposing a simple-to-follow workflow based on an ensemble of widely available physico-chemical technologies, to assess sequentially the essential properties of any protein sample: purity and integrity, homogeneity and activity. Approaches are then suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency.
Document type :
Journal articles
Complete list of metadata

Cited literature [73 references]  Display  Hide  Download
Contributor : Patrick ENGLAND Connect in order to contact the contributor
Submitted on : Thursday, January 22, 2015 - 6:33:02 AM
Last modification on : Thursday, April 7, 2022 - 10:10:30 AM
Long-term archiving on: : Thursday, April 23, 2015 - 10:11:04 AM


Protein quality control review...
Publisher files allowed on an open archive




Bertrand Raynal, Pascal Lenormand, Bruno Baron, Sylviane Hoos, Patrick England. Quality assessment and optimization of purified protein samples: why and how?. Microbial Cell Factories, 2014, 13, pp.180. ⟨10.1186/s12934-014-0180-6⟩. ⟨pasteur-01108005⟩



Record views


Files downloads