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The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest.

Gotthard Kunze 1, 2, * Claude Gaillardin 3 Małgorzata Czernicka 4 Pascal Durrens 5, 6 Tiphaine Martin 5, 6 Erik Böer 1 Toni Gabaldón 7, 8 Jose Cruz 9 Emmanuel Talla 10 Christian Marck 11 André Goffeau 12 Valérie Barbe 13 Philippe Baret 14 Keith Baronian 15 Sebastian Beier 1 Claudine Bleykasten 9 Rüdiger Bode 16 Serge Casaregola 3 Laurence Despons 17 Cécile Fairhead 18 Martin Giersberg 1 Przemysław Piotr Gierski 19 Urs Hähnel 1 Anja Hartmann 1 Dagmara Jankowska 1 Claire Jubin 20 Paul Jung 21 Ingrid Lafontaine 22 Véronique Leh-Louis 21 Marc Lemaire 23 Marina Marcet-Houben 7, 8 Martin Mascher 1 Guillaume Morel 3 Guy-Franck Richard 22 Jan Riechen 1 Christine Sacerdot 22 Anasua Sarkar 5 Guilhem Savel 5 Joseph Schacherer 21 David Sherman 5, 6 Nils Stein 1 Marie-Laure Straub 21 Agnès Thierry 22 Anke Trautwein-Schult 1 Benoit Vacherie 13 Eric Westhof 9 Sebastian Worch 1 Bernard Dujon 22 Jean-Luc Souciet 21 Patrick Wincker 13 Uwe Scholz 1 Cécile Neuvéglise 3, *
Abstract : BACKGROUND: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. RESULTS: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. CONCLUSIONS: The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.
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Submitted on : Thursday, May 8, 2014 - 9:20:33 PM
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Gotthard Kunze, Claude Gaillardin, Małgorzata Czernicka, Pascal Durrens, Tiphaine Martin, et al.. The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest.. Biotechnology for Biofuels, BioMed Central, 2014, 7 (1), pp.66. ⟨10.1186/1754-6834-7-66⟩. ⟨pasteur-00988609⟩

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