The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest.

Gotthard Kunze (1, 2) , Claude Gaillardin (3) , Małgorzata Czernicka (4) , Pascal Durrens (5, 6) , Tiphaine Martin (5, 6) , Erik Böer (1) , Toni Gabaldón (7, 8) , Jose Cruz (9) , Emmanuel Talla (10) , Christian Marck (11) , André Goffeau (12) , Valérie Barbe (13) , Philippe Baret (14) , Keith Baronian (15) , Sebastian Beier (1) , Claudine Bleykasten (9) , Rüdiger Bode (16) , Serge Casaregola (3) , Laurence Despons (17) , Cécile Fairhead (18) , Martin Giersberg (1) , Przemysław Piotr Gierski (19) , Urs Hähnel (1) , Anja Hartmann (1) , Dagmara Jankowska (1) , Claire Jubin (20) , Paul Jung (21) , Ingrid Lafontaine (22) , Véronique Leh-Louis (21) , Marc Lemaire (23) , Marina Marcet-Houben (7, 8) , Martin Mascher (1) , Guillaume Morel (3) , Guy-Franck Richard (22) , Jan Riechen (1) , Christine Sacerdot (22) , Anasua Sarkar (5) , Guilhem Savel (5) , Joseph Schacherer (21) , David Sherman (5, 6) , Nils Stein (1) , Marie-Laure Straub (21) , Agnès Thierry (22) , Anke Trautwein-Schult (1) , Benoit Vacherie (13) , Eric Westhof (9) , Sebastian Worch (1) , Bernard Dujon (22) , Jean-Luc Souciet (21) , Patrick Wincker (13) , Uwe Scholz (1) , Cécile Neuvéglise (3)

1 IPK-Gatersleben - Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben]
2 Yeast Genetics
3 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
4 Institute of Plant Biology and Biotechnology
5 LaBRI - Laboratoire Bordelais de Recherche en Informatique
6 MAGNOME - Models and Algorithms for the Genome
7 Bioinformatics and Genomics Programme
8 UPF - Universitat Pompeu Fabra [Barcelona]
9 ARN - Architecture et Réactivité de l'ARN
10 LCB - Laboratoire de chimie bactérienne
11 IBITECS - Institut de Biologie et de Technologies de Saclay
12 UCL - Université Catholique de Louvain = Catholic University of Louvain
13 IG - Institut de Génomique d'Evry
14 ELI - Earth and Life Institute [Louvain-La-Neuve]
15 School of Biological Sciences
16 Institute of Biochemistry
17 UNISTRA - Université de Strasbourg
18 IGM - Institut de génétique et microbiologie [Orsay]
19 Laboratory of Bioinformatics and Protein Engineering
20 UMR 8030 - Génomique métabolique
21 GMGM - Génétique moléculaire, génomique, microbiologie
22 Génétique Moléculaire des Levures
23 YMG - Génétique moléculaire des levures

BACKGROUND: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. RESULTS: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. CONCLUSIONS: The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.

Keywords: yeast; genome; tannic acid; metabolism

Keywords: n-butanol; biotechnology

Domains: Life Sciences [q-bio]; Biotechnology; Computer Science [cs]; Biotechnology

Gotthard Kunze, Claude Gaillardin, Małgorzata Czernicka, Pascal Durrens, Tiphaine Martin, et al.. The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest.. Biotechnology for Biofuels, 2014, 7 (1), pp.66. ⟨10.1186/1754-6834-7-66⟩. ⟨pasteur-00988609⟩