Origin, trafficking, and intraepithelial fate of gut-tropic T cells. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Medicine Année : 2013

Origin, trafficking, and intraepithelial fate of gut-tropic T cells.

Résumé

The small intestine epithelium (SI-Ep) harbors millions of unconventional (γδ and CD4(-) CD8(-) NK1.1(-) TCRαβ) and conventional (CD8αβ and CD4) T cells, designated intraepithelial lymphocytes (IELs). Here, we identified the circulating pool of SI-Ep-tropic T cells and studied their capacity to colonize the SI-Ep under steady-state conditions in SPF mice. Developmentally regulated levels of α4β7 endowed recent thymic emigrants (RTEs) of unconventional types with higher SI-Ep tropism than their conventional homologues. SI-Ep-tropic RTEs, which in all lineages emerged naive, homed to the SI-Ep, but this environment was inadequate to stimulate them to cycle. In contrast, conventional and, unexpectedly, unconventional T cells, particularly Vγ7(+) (hallmark of γδ IELs), previously stimulated to cycle in the gut-associated lymphoid tissue (GALT), proliferated in the SI-Ep. Cycling unconventional SI-Ep immigrants divided far more efficiently than their conventional homologues, thereby becoming predominant. This difference impacted on acquisition of high Granzyme B content, which required extensive proliferation. In conclusion, SI-Ep-tropic T cells follow a thymus-SI-Ep or a GALT-SI-Ep pathway, the latter generating highly competitive immigrants that are the sole precursors of cytotoxic IELs. These events occur continuously as part of the normal IEL dynamics.

Domaines

Immunologie
Fichier principal
Vignette du fichier
J_Exp_Med-2013-Guy-Grand-1839-54.pdf (2.53 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

pasteur-00854430 , version 1 (02-03-2014)

Identifiants

Citer

Delphine Guy-Grand, Pierre Vassalli, Gerard Eberl, Pablo Pereira, Odile Burlen-Defranoux, et al.. Origin, trafficking, and intraepithelial fate of gut-tropic T cells.. Journal of Experimental Medicine, 2013, 210 (9), pp.1839-54. ⟨10.1084/jem.20122588⟩. ⟨pasteur-00854430⟩
231 Consultations
512 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More