Abstract : A large number of nucleoside analogs and 2'- deoxynucleoside triphosphates (dNTPs) have been synthesized in order to interfere with DNA metabolism. However, in vivo the concentration and phosphorylation of these analogues are key limiting factors. In this context, we designed enzymes to switch nucleobases attached to a deoxyribose monophosphate. Active chimeras were made from two distantly related enzymes: a nucleoside deoxyribosyltransferase (NDT) from lactobacilli and a 5'-monophosphate-2'-deoxyribonucleoside hydrolase (Rcl) from rat. Then, their unprecedented activity was further extended to deoxyribose triphosphate and, in vitro biosyntheses could be successfully performed with several base analogues. These new enzymes provide new tools to synthesize dNTPs analogs and to deliver them into cells.
https://hal-pasteur.archives-ouvertes.fr/pasteur-00796440
Contributor : Alexandre Kaminski <>
Submitted on : Monday, March 4, 2013 - 10:59:08 AM Last modification on : Monday, January 11, 2021 - 2:30:05 PM Long-term archiving on: : Sunday, April 2, 2017 - 8:40:16 AM
File
Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed
until : jamais
Pierre Alexandre Kaminski, Gilles Labesse. Phosphodeoxyribosyltransferases: designed enzymes for deoxyribonucleotide synthesis.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2013, 288 (9), pp.6534-6541. ⟨10.1074/jbc.M112.446492⟩. ⟨pasteur-00796440⟩