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Journal articles
RANKL induces organized lymph node growth by stromal cell proliferation.
Estelle Hess
1
Vincent Duheron
1
Marion Decossas
1
Frédéric Lézot
2, 3
Ariane Berdal
2
Sylvestre Chea
4
Rachel Golub
4
Mattéo R Bosisio
5, 6
S Lori Bridal
5
Yongwon Choi
7
Hideo Yagita
8
Christopher G Mueller
1, *
*
Corresponding author
S Lori Bridal 5
Author IdHAL : sharon-bridal ORCID : https://orcid.org/0000-0001-5053-5423
Yongwon Choi 7
Author
Hideo Yagita 8
Author
Christopher G Mueller 1
Correspondent author IdHAL : christopher-mueller ORCID : https://orcid.org/0000-0002-4119-2729
1
ICT - Immunologie et chimie thérapeutiques (I.B.M.C. 15 Rue René Descartes 67084 STRASBOURG CEDEX - France)
- Cancéropôle du Grand Est (France)
- CNRS - Centre National de la Recherche Scientifique : UPR9021 (France)
2
CRC (UMR_S 872) - Centre de Recherche des Cordeliers (CRBM des Cordeliers 15, rue de l'ecole de medecine batiment E 75270 Paris cedex 06 - France)
- UPD5 - Université Paris Descartes - Paris 5 : U872 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U872 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 872 (France)
3
PhAN - Physiopathologie des Adaptations Nutritionnelles (1 place Alexis Ricordeau 44093 Nantes Cedex 1 France - France)
- UFR MEDECINE - Université de Nantes - UFR de Médecine et des Techniques Médicales (1 rue Gaston Veil BP 53508 44035 NANTES Cedex 1 - France)
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
- INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : UMR_A1280 (France)
4
Lymphopoïèse (25-28, rue du Docteur Roux 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U668 (101, rue de Tolbiac, 75013 Paris - France)
5
LIP - Laboratoire d'Imagerie Paramétrique (15 Rue de l'école de Médecine 75006 PARIS - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- IFR58 (France)
- CNRS - Centre National de la Recherche Scientifique : UMR7623 (France)
7
Department of Pathology and Laboratory Medicine (University of Pennsylvania School of Medicine Philadelphia, 19104 - United States)
- University of Pennsylvania [Philadelphia] (3451 Walnut Street, Philadelphia, PA 19104 | 215-898-5000 - United States)
8
Department of Immunology (Jutendo University School of Medicine Tokyo 113-8421 - Japan)
Abstract : RANK and its ligand RANKL play important roles in the development and regulation of the immune system. We show that mice transgenic for Rank in hair follicles display massive postnatal growth of skin-draining lymph nodes. The proportions of hematopoietic and nonhematopoietic stromal cells and their organization are maintained, with the exception of an increase in B cell follicles. The hematopoietic cells are not activated and respond to immunization by foreign Ag and adjuvant. We demonstrate that soluble RANKL is overproduced from the transgenic hair follicles and that its neutralization normalizes lymph node size, inclusive area, and numbers of B cell follicles. Reticular fibroblastic and vascular stromal cells, important for secondary lymphoid organ formation and organization, express RANK and undergo hyperproliferation, which is abrogated by RANKL neutralization. In addition, they express higher levels of CXCL13 and CCL19 chemokines, as well as MAdCAM-1 and VCAM-1 cell-adhesion molecules. These findings highlight the importance of tissue-derived cues for secondary lymphoid organ homeostasis and identify RANKL as a key molecule for controlling the plasticity of the immune system.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00782725
Contributor : Marie-Christine Vougny <>
Submitted on : Wednesday, January 30, 2013 - 2:46:51 PM
Last modification on : Tuesday, September 28, 2021 - 3:24:49 AM
Contributor : Marie-Christine Vougny <>
Submitted on : Wednesday, January 30, 2013 - 2:46:51 PM
Last modification on : Tuesday, September 28, 2021 - 3:24:49 AM
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- HAL Id : pasteur-00782725, version 1
- DOI : 10.4049/jimmunol.1101513
- PUBMED : 22210913
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UNIV-NANTES | CNRS | CGE | UNIV-PARIS5 | CORDELIERS | LIB | UPMC | ATP-IMMUN | PASTEUR | USPC | SORBONNE-UNIVERSITE | SU-SCIENCES | UNIV-PARIS | UP-SCIENCES | SU-TI | INRAE
Citation
Estelle Hess, Vincent Duheron, Marion Decossas, Frédéric Lézot, Ariane Berdal, et al.. RANKL induces organized lymph node growth by stromal cell proliferation.. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2012, 188 (3), pp.1245-54. ⟨10.4049/jimmunol.1101513⟩. ⟨pasteur-00782725⟩
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