RANKL induces organized lymph node growth by stromal cell proliferation.

Estelle Hess; Vincent Duheron; Marion Decossas; Frédéric Lézot; Ariane Berdal; Sylvestre Chea; Rachel Golub; Mattéo R Bosisio; S Lori Bridal; Yongwon Choi; Hideo Yagita; Christopher G Mueller

doi:10.4049/jimmunol.1101513

Journal articles

RANKL induces organized lymph node growth by stromal cell proliferation.

Estelle Hess ¹ Vincent Duheron ¹ Marion Decossas ¹ Frédéric Lézot ^{2, 3} Ariane Berdal ² Sylvestre Chea ⁴ Rachel Golub ⁴ Mattéo R Bosisio ^{5, 6} S Lori Bridal ⁵ Yongwon Choi ⁷ Hideo Yagita ⁸ Christopher G Mueller ^{1, *}

* Corresponding author

1 ICT - Immunologie et chimie thérapeutiques

2 CRC (UMR_S 872) - Centre de Recherche des Cordeliers

3 PhAN - Physiopathologie des Adaptations Nutritionnelles

4 Lymphopoïèse

5 LIP - Laboratoire d'Imagerie Paramétrique

6 ECHOSENS S.A.

7 Department of Pathology and Laboratory Medicine

8 Department of Immunology

Abstract : RANK and its ligand RANKL play important roles in the development and regulation of the immune system. We show that mice transgenic for Rank in hair follicles display massive postnatal growth of skin-draining lymph nodes. The proportions of hematopoietic and nonhematopoietic stromal cells and their organization are maintained, with the exception of an increase in B cell follicles. The hematopoietic cells are not activated and respond to immunization by foreign Ag and adjuvant. We demonstrate that soluble RANKL is overproduced from the transgenic hair follicles and that its neutralization normalizes lymph node size, inclusive area, and numbers of B cell follicles. Reticular fibroblastic and vascular stromal cells, important for secondary lymphoid organ formation and organization, express RANK and undergo hyperproliferation, which is abrogated by RANKL neutralization. In addition, they express higher levels of CXCL13 and CCL19 chemokines, as well as MAdCAM-1 and VCAM-1 cell-adhesion molecules. These findings highlight the importance of tissue-derived cues for secondary lymphoid organ homeostasis and identify RANKL as a key molecule for controlling the plasticity of the immune system.

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Journal articles

Domain :

Life Sciences [q-bio] / Immunology

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Submitted on : Wednesday, January 30, 2013 - 2:46:51 PM
Last modification on : Wednesday, January 26, 2022 - 3:04:15 AM

RANKL induces organized lymph node growth by stromal cell proliferation.

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