Skip to Main content Skip to Navigation
New interface
Journal articles

Amyloid Fibrils Formed by the Programmed Cell Death Regulator Bcl-xL.: Amyloid Fibrils from Bcl-xL

Abstract : The accumulation of amyloid fibers due to protein misfolding is associated with numerous human diseases. For example, the formation of amyloid deposits in neurodegenerative pathologies is correlated with abnormal apoptosis. We report here the in vitro formation of various types of aggregates by Bcl-xL, a protein of the Bcl-2 family involved in the regulation of apoptosis. Bcl-xL forms aggregates in three states, micelles, native-like fibrils, and amyloid fibers, and their biophysical characterization has been performed in detail. Bcl-xL remains in its native state within micelles and native-like fibrils, and our results suggest that native-like fibrils are formed by the association of micelles. Formation of amyloid structures, that is, nonnative intermolecular β-sheets, is favored by the proximity of proteins within fibrils at the expense of the Bcl-xL native structure. Finally, we provide evidence of a direct relationship between the amyloid character of the fibers and the tertiary-structure stability of the native Bcl-xL. The potential causality between the accumulation of Bcl-xL into amyloid deposits and abnormal apoptosis during neurodegenerative diseases is discussed.
Document type :
Journal articles
Complete list of metadata

Cited literature [80 references]  Display  Hide  Download
Contributor : Alexandre Chenal Connect in order to contact the contributor
Submitted on : Thursday, December 22, 2011 - 9:21:16 PM
Last modification on : Wednesday, October 5, 2022 - 11:12:07 AM
Long-term archiving on: : Monday, December 5, 2016 - 10:05:31 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document



Alexandre Chenal, Charlotte Vendrely, Heidi Vitrac, Johanna C Karst, Alexis Gonneaud, et al.. Amyloid Fibrils Formed by the Programmed Cell Death Regulator Bcl-xL.: Amyloid Fibrils from Bcl-xL. Journal of Molecular Biology, 2011, epub ahead of print. ⟨10.1016/j.jmb.2011.11.024⟩. ⟨pasteur-00654783⟩



Record views