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Journal articles
Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation.
Abstract : Antigen recognition within immunological synapses triggers and sustains T cell activation by nucleating protein microclusters that gather T cell receptors (TCRs), kinases, and adaptors. Dissipation of these microclusters results in signal termination, but how this process is regulated is unclear. In this paper, we reveal that release of the adaptors SLP76 and GADS from signaling microclusters is induced by the serine/threonine protein kinase HPK1 and that phosphorylation of GADS plays a major role in this process. We found that HPK1 was recruited into microclusters and triggered their dissipation by inducing the phosphorylation of a threonine-containing motif of GADS, together with the previously described serine phosphorylation of SLP76. These events induced the cooperative binding of 14-3-3 proteins to SLP76-GADS complexes, leading to their uncoupling from the transmembrane adaptor LAT and consequently reducing microcluster persistence and activation-induced gene transcription. These results demonstrate that serine/threonine phosphorylation of multiple TCR-proximal effectors controls the stability of signaling microclusters, thereby determining the intensity of T cell responses.
Cited literature [35 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-00646203
Contributor : Vincenzo Di Bartolo <>
Submitted on : Tuesday, May 29, 2012 - 7:00:05 AM
Last modification on : Thursday, October 22, 2020 - 3:12:05 PM
Long-term archiving on: : Thursday, August 30, 2012 - 2:16:48 AM
Contributor : Vincenzo Di Bartolo <>
Submitted on : Tuesday, May 29, 2012 - 7:00:05 AM
Last modification on : Thursday, October 22, 2020 - 3:12:05 PM
Long-term archiving on: : Thursday, August 30, 2012 - 2:16:48 AM
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