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Journal articles
Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.
Rémi Lasserre
1
Stephanie Charrin
1
Céline Cuche
1
Anne Danckaert
2
Maria-Isabel Thoulouze
1
Fabrice de Chaumont
3
Tarn Duong
4
Nathalie Perrault
5
Nadine Varin-Blank
5
Jean-Christophe Olivo-Marin
3
Sandrine Etienne-Manneville
6
Monique Arpin
7
Vincenzo Di Bartolo
1
Andrès Alcover
1, *
Maria-Isabel Thoulouze 1
Author
Fabrice de Chaumont 3
Author
Tarn Duong 4
Author
Nathalie Perrault 5
Author
Nadine Varin-Blank 5
Author
Jean-Christophe Olivo-Marin 3
Author
Sandrine Etienne-Manneville 6
Author
Monique Arpin 7
Author
Vincenzo Di Bartolo 1
Author IdHAL : vincenzo-di-bartolo ORCID : https://orcid.org/0000-0002-5453-947X
Andrès Alcover 1
Correspondent author
1
BIOCELLY - Biologie Cellulaire des Lymphocytes (25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA1961 (France)
2
PFID - Imagerie Dynamique (Plate-Forme) (25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
3
AIQ - Analyse d'Images Quantitative (25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA2582 (France)
4
Imagerie et Modélisation (28, rue du Docteur Roux 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA2582 (France)
5
UMR_S567 / UMR 8104 - Institut Cochin (Bâtiment MECHAIN 22 rue Méchain 75014 PARIS - France)
- UPD5 - Université Paris Descartes - Paris 5 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U567 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8104 (France)
6
Polarité et Migration Cellulaires (25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA2582 (France)
7
Morphogénèse et Signalisation Cellulaires (25 rue d'Ulm 75005 Paris - France)
- Institut Curie [Paris] ( 26 rue d'Ulm - 75248 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR144 (France)
Abstract : T-cell receptor (TCR) signalling is triggered and tuned at immunological synapses by the generation of signalling complexes that associate into dynamic microclusters. Microcluster movement is necessary to tune TCR signalling, but the molecular mechanism involved remains poorly known. We show here that the membrane-microfilament linker ezrin has an important function in microcluster dynamics and in TCR signalling through its ability to set the microtubule network organization at the immunological synapse. Importantly, ezrin and microtubules are important to down-regulate signalling events leading to Erk1/2 activation. In addition, ezrin is required for appropriate NF-AT activation through p38 MAP kinase. Our data strongly support the notion that ezrin regulates immune synapse architecture and T-cell activation through its interaction with the scaffold protein Dlg1. These results uncover a crucial function for ezrin, Dlg1 and microtubules in the organization of the immune synapse and TCR signal down-regulation. Moreover, they underscore the importance of ezrin and Dlg1 in the regulation of NF-AT activation through p38.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00593159
Contributor : Louise Da <>
Submitted on : Thursday, September 24, 2020 - 9:25:50 AM
Last modification on : Friday, October 16, 2020 - 2:55:34 PM
Contributor : Louise Da <>
Submitted on : Thursday, September 24, 2020 - 9:25:50 AM
Last modification on : Friday, October 16, 2020 - 2:55:34 PM
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Identifiers
- HAL Id : pasteur-00593159, version 1
- DOI : 10.1038/emboj.2010.127
- PRODINRA : 481173
- PUBMED : 20551903
Collections
PASTEUR | UP-SANTE | UNIV-PARIS | SU-SCIENCES | SORBONNE-UNIVERSITE | USPC | PSL | ATP-IMMUN | UMR144 | CURIE | FNCLCC | CNRS | UNIV-PARIS5
Citation
Rémi Lasserre, Stephanie Charrin, Céline Cuche, Anne Danckaert, Maria-Isabel Thoulouze, et al.. Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.. EMBO Journal, EMBO Press, 2010, 29 (14), pp.2301-14. ⟨10.1038/emboj.2010.127⟩. ⟨pasteur-00593159⟩
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