Skip to Main content Skip to Navigation
Book sections

dsRNA uptake in adult Drosophila

Abstract : RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans. In this chapter, we describe how to introduce exogenous dsRNA into adult Drosophila in order to interfere with endogenous or viral gene expression.
Document type :
Book sections
Complete list of metadatas

Cited literature [22 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-00591080
Contributor : Mireille Gau <>
Submitted on : Thursday, January 31, 2019 - 11:20:03 AM
Last modification on : Tuesday, October 13, 2020 - 12:22:02 PM
Long-term archiving on: : Wednesday, May 1, 2019 - 2:43:14 PM

File

Ch16 Obadia Saleh Final.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Benjamin Obadia, Maria-Carla Saleh. dsRNA uptake in adult Drosophila. van Rij R. Methods in Molecular Biology, 721, pp.253-63, 2011, Antiviral RNAi : Concepts, Methods, and Applications, 978-1-61779-037-9. ⟨10.1007/978-1-61779-037-9_16⟩. ⟨pasteur-00591080⟩

Share

Metrics

Record views

283

Files downloads

424