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Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.

Abstract : We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5'ArA and 5'UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.
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Submitted on : Monday, January 10, 2011 - 4:05:17 PM
Last modification on : Friday, June 19, 2020 - 1:32:02 AM

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Rodolphe Suspène, Vincent Petit, David Puyraimond-Zemmour, Marie-Ming Aynaud, Michel Henry, et al.. Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.. Journal of Virology, American Society for Microbiology, 2011, 85 (5), pp.2458-62. ⟨10.1128/JVI.02138-10⟩. ⟨pasteur-00554335⟩

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