Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.

Rodolphe Suspène (1) , Vincent Petit (1) , David Puyraimond-Zemmour (1) , Marie-Ming Aynaud (1) , Michel Henry (1) , Denise Guétard (1) , Christophe Rusniok (2) , Simon Wain-Hobson (1) , Jean Pierre Vartanian (1)

1 Rétrovirologie Moléculaire
2 Biologie des bactéries intracellulaires

We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5'ArA and 5'UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.

Domaines: Microbiologie et Parasitologie

Rodolphe Suspène, Vincent Petit, David Puyraimond-Zemmour, Marie-Ming Aynaud, Michel Henry, et al.. Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.. Journal of Virology, 2011, 85 (5), pp.2458-62. ⟨10.1128/JVI.02138-10⟩. ⟨pasteur-00554335⟩