 |
Journal articles
Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.
Abstract : We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5'ArA and 5'UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.
https://hal-pasteur.archives-ouvertes.fr/pasteur-00554335
Contributor : Mireille Gau Connect in order to contact the contributor
Submitted on : Monday, January 10, 2011 - 4:05:17 PM
Last modification on : Thursday, January 13, 2022 - 6:32:02 PM
Contributor : Mireille Gau Connect in order to contact the contributor
Submitted on : Monday, January 10, 2011 - 4:05:17 PM
Last modification on : Thursday, January 13, 2022 - 6:32:02 PM
Links full text
