 |
Journal articles
Impact of peptidoglycan O-acetylation on autolytic activities of the Enterococcus faecalis N-acetylglucosaminidase AtlA and N-acetylmuramidase AtlB.
Abstract : Autolysins are potentially lethal enzymes that partially hydrolyze peptidoglycan for incorporation of new precursors and septum cleavage after cell division. Here, we explored the impact of peptidoglycan O-acetylation on the enzymatic activities of Enterococcus faecalis major autolysins, the N-acetylglucosaminidase AtlA and the N-acetylmuramidase AtlB. We constructed isogenic strains with various O-acetylation levels and used them as substrates to assay E. faecalis autolysin activities. Peptidoglycan O-acetylation had a marginal inhibitory impact on the activities of these enzymes. In contrast, removal of cell wall glycopolymers increased the AtlB activity (37-fold), suggesting that these polymers negatively control the activity of this enzyme.
https://hal-pasteur.archives-ouvertes.fr/pasteur-00512062
Contributor : Cécile Roux <>
Submitted on : Friday, August 27, 2010 - 11:29:22 AM
Last modification on : Wednesday, May 26, 2021 - 8:08:02 PM
Contributor : Cécile Roux <>
Submitted on : Friday, August 27, 2010 - 11:29:22 AM
Last modification on : Wednesday, May 26, 2021 - 8:08:02 PM