Biofilm-like extracellular viral assemblies mediate HTLV-1 cell-to-cell transmission at virological synapses.

Ana-Monica Pais-Correia 1 Martin Sachse 2 Stéphanie Guadagnini 2 Valentina Robbiati 1 Rémi Lasserre 1 Antoine Gessain 3 Olivier Gout 4 Andrés Alcover 1, * Maria-Isabel Thoulouze 1, *
* Corresponding author
1 BIOCELLY - Biologie Cellulaire des Lymphocytes
2 CITECH - Imagopole
3 Epidémiologie et Physiopathologie des Virus Oncogènes
4 Service de Neurologie
Abstract : Human T cell leukemia virus type 1 (HTLV-1) is a lymphotropic retrovirus whose cell-to-cell transmission requires cell contacts. HTLV-1-infected T lymphocytes form 'virological synapses', but the mechanism of HTLV-1 transmission remains poorly understood. We show here that HTLV-1-infected T lymphocytes transiently store viral particles as carbohydrate-rich extracellular assemblies that are held together and attached to the cell surface by virally-induced extracellular matrix components, including collagen and agrin, and cellular linker proteins, such as tetherin and galectin-3. Extracellular viral assemblies rapidly adhere to other cells upon cell contact, allowing virus spread and infection of target cells. Their removal strongly reduces the ability of HTLV-1-producing cells to infect target cells. Our findings unveil a novel virus transmission mechanism based on the generation of extracellular viral particle assemblies whose structure, composition and function resemble those of bacterial biofilms. HTLV-1 biofilm-like structures represent a major route for virus transmission from cell to cell.
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Journal articles
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Submitted on : Friday, February 26, 2010 - 12:35:12 PM
Last modification on : Friday, September 21, 2018 - 4:42:02 PM

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Ana-Monica Pais-Correia, Martin Sachse, Stéphanie Guadagnini, Valentina Robbiati, Rémi Lasserre, et al.. Biofilm-like extracellular viral assemblies mediate HTLV-1 cell-to-cell transmission at virological synapses.. Nature Medicine, Nature Publishing Group, 2010, 16 (1), pp.83-9. ⟨10.1038/nm.2065⟩. ⟨pasteur-00460124⟩

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