CrAsH-quantum dot nanohybrids for smart targeting of proteins.

Emilie Genin (1) , Olivier Carion (2) , Benoit Mahler (2) , Benoit Dubertret (2) , Nathalie Arhel (3) , Pierre Charneau (3) , Eric Doris (1) , Charles Mioskowski (1)

1 SCBM - Service de Chimie Bio-Organique et de Marquage
2 Laboratoire Photons Et Matière
3 Virologie moléculaire et Vectorologie

Smart nanohybrids were prepared by conjugation of CrAsH to hydrosoluble and biocompatible quantum dots (QDs). The resulting probes were shown to bind efficiently and selectively to Cys-tagged proteins. The interaction with the protein was detected by an increase of the fluorescence emission of CrAsH. While the latter faded rapidly under continuous excitation, emission of the QD remained unaffected. The persistent fluorescence of the QD should thus allow extended monitoring of the target protein.

Domains: Microbiology and Parasitology

Emilie Genin, Olivier Carion, Benoit Mahler, Benoit Dubertret, Nathalie Arhel, et al.. CrAsH-quantum dot nanohybrids for smart targeting of proteins.. Journal of the American Chemical Society, 2008, 130 (27), pp.8596-7. ⟨10.1021/ja802987q⟩. ⟨pasteur-00457766⟩