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Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus.

Abstract : The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of this RNA virus, we determined the three-dimensional (3D) crystal structure at 3.3 A resolution of a decameric, annular ribonucleoprotein complex of the RSV nucleoprotein (N) bound to RNA. This complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral RNA synthesis. The RNA wraps around the protein ring, with seven nucleotides contacting each N subunit, alternating rows of four and three stacked bases that are exposed and buried within a protein groove, respectively. Combined with electron microscopy data, this structure provides a detailed model for the RSV nucleocapsid, in which the bases are accessible for readout by the viral polymerase. Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00457523
Contributor : Mireille Gau <>
Submitted on : Wednesday, February 17, 2010 - 3:27:04 PM
Last modification on : Wednesday, September 16, 2020 - 5:05:42 PM

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Rajiv G. Tawar, Stéphane Duquerroy, Clemens Vonrhein, Paloma F. Varela, Laurence Damier-Piolle, et al.. Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus.. Science, American Association for the Advancement of Science, 2009, 326 (5957), pp.1279-83. ⟨10.1126/science.1177634⟩. ⟨pasteur-00457523⟩

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