Hepatitis C virus infection protein network.

B. de Chassey (1, 2) , V. Navratil (2, 3) , L. Tafforeau (1, 2) , M. S. Hiet (1, 2) , A. Aublin-Gex (1, 2) , S. Agaugué (1, 2) , G. Meiffren (1, 2) , F. Pradezynski (1, 2) , B. F. Faria (1, 2) , T. Chantier (1, 2) , M. Le Breton (1, 2) , J. Pellet (1, 2) , N. Davoust (1, 2) , P. E. Mangeot (1, 2) , A. Chaboud (2, 4) , F. Penin (2, 4) , Y. Jacob (5) , P. O. Vidalain (6) , M. Vidal (7) , P. André (1, 2, 8) , C. Rabourdin-Combe (1, 2) , V. Lotteau (2, 8)

1 I2V - Immunité infection vaccination
2 BLG - BioSciences Lyon-Gerland
3 RPC - Rétrovirus et Pathologie Comparée
4 IBCP - Institut de biologie et chimie des protéines [Lyon]
5 Génétique, Papillomavirus et Cancer Humain
6 Génomique Virale et Vaccination
7 CCSB - Center for Cancer Systems Biology
8 Laboratoire de virologie

A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.

Keywords: FUNCTIONAL ANALYSIS; HEPATITIS C; INTERACTOME; VIRUS–HOST CELL

Domains: Microbiology and Parasitology

B. de Chassey, V. Navratil, L. Tafforeau, M. S. Hiet, A. Aublin-Gex, et al.. Hepatitis C virus infection protein network.. Molecular Systems Biology, 2008, 4 (230), pp.230. ⟨10.1038/msb.2008.66⟩. ⟨pasteur-00456466⟩