ViralORFeome: an integrated database to generate a versatile collection of viral ORFs. - Archive ouverte HAL Access content directly
Journal Articles Nucleic Acids Research Year : 2010

ViralORFeome: an integrated database to generate a versatile collection of viral ORFs.

(1) , (1) , (2) , (3, 4) , (1) , (5) , (1, 6) , (1) , (2) , (7) , (4, 8) , (1) , (1) , (7) , (1) , (7) , (5) , (5) , (4, 6) , (4, 9) , (4, 10) , (4, 10) , (7) , (2) , (7) , (1, 4) , (1) , (1) , (2)
1
2
3
4
5
6
7
8
9
10
Guillaume Achaz
  • Function : Author
  • PersonId : 863418
M. Le Breton
L. Jacotot
  • Function : Author
P. Vaglio
  • Function : Author

Abstract

Large collections of protein-encoding open reading frames (ORFs) established in a versatile recombination-based cloning system have been instrumental to study protein functions in high-throughput assays. Such 'ORFeome' resources have been developed for several organisms but in virology, plasmid collections covering a significant fraction of the virosphere are still needed. In this perspective, we present ViralORFeome 1.0 (http://www.viralorfeome.com), an open-access database and management system that provides an integrated set of bioinformatic tools to clone viral ORFs in the Gateway(R) system. ViralORFeome provides a convenient interface to navigate through virus genome sequences, to design ORF-specific cloning primers, to validate the sequence of generated constructs and to browse established collections of virus ORFs. Most importantly, ViralORFeome has been designed to manage all possible variants or mutants of a given ORF so that the cloning procedure can be applied to any emerging virus strain. A subset of plasmid constructs generated with ViralORFeome platform has been tested with success for heterologous protein expression in different expression systems at proteome scale. ViralORFeome should provide our community with a framework to establish a large collection of virus ORF clones, an instrumental resource to determine functions, activities and binding partners of viral proteins.
Fichier principal
Vignette du fichier
D371.pdf (9.17 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

pasteur-00455342 , version 1 (11-02-2010)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

J. Pellet, L. Tafforeau, M. Lucas-Hourani, V. Navratil, L. Meyniel, et al.. ViralORFeome: an integrated database to generate a versatile collection of viral ORFs.. Nucleic Acids Research, 2010, 38 (Issue suppl1: Database issue), pp.D371-8. ⟨10.1093/nar/gkp1000⟩. ⟨pasteur-00455342⟩
505 View
333 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More