TCR-inducible PLZF transcription factor required for innate phenotype of a subset of gammadelta T cells with restricted TCR diversity.

Taras Kreslavsky (1) , Adam K. Savage (2) , Robin Hobbs (3) , Fotini Gounari (4) , Roderick Bronson (5) , Pablo Pereira (6) , Pier Paolo Pandolfi (3) , Albert Bendelac (2) , Harald von Boehmer (1)

1 Laboratory of Lymphocyte Biology
2 Committe on Immunology
3 Cancer Genetics Program
4 Department of Medicine
5 Rodent Histopathology Laboratory
6 Développement des Lymphocytes

Some gammadelta and alphabeta T lymphocytes exhibit an "innate" phenotype associated with rapid cytokine responses. The PLZF transcription factor is essential for the innate phenotype of NKT cells. This report shows that PLZF is likewise responsible for the innate, NKT-like phenotype of Vgamma1+Vdelta6.3/Vdelta6.4+ cells. TCR cross-linking induced PLZF expression in all polyclonal immature gammadelta thymocytes, suggesting that agonist selection might be required for PLZF induction. Transgenic expression of Vgamma1Vdelta6.4 TCR was sufficient to support the development of large numbers of PLZF+ T cells, further supporting the importance of the TCR for PLZF induction. Interestingly, expression of this TCR transgene led to the development of spontaneous dermatitis.

Domaines: Immunologie

Taras Kreslavsky, Adam K. Savage, Robin Hobbs, Fotini Gounari, Roderick Bronson, et al.. TCR-inducible PLZF transcription factor required for innate phenotype of a subset of gammadelta T cells with restricted TCR diversity.. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106 (30), pp.12453-8. ⟨10.1073/pnas.0903895106⟩. ⟨pasteur-00429047⟩