K. Korth, Germany) were cultured in DMEM with addition of 10% FCS (Invitrogen) ScCAD cells infected with 139A prion strain (gift of Dr Jouy-en-Josas, France) were cultured in DMEM:F12 (Invitrogen) with addition of 10% FBS. U18666A was purchased from Calbiochem. All other chemicals were purchased from Sigma. SAF32 and SAF61 monoclonal antibodies were purchased from SPI-BIO, while POM1 monoclonal antibodies were purchased from Dr Anti-tubulin monoclonal antibodies were from Sigma. Anti-flotillin-1 monoclonal antibodies were from BD Transduction Laboratories. Anti-GFP antibodies and all the fluorescently labeled secondary antibodies were purchased from Invitrogen (Molecular Probes), as well as Lysosensor, fluorescently tagged transferrin and dextran. Anti-EEA-1 antibodies were kind gift of Dr Anti-Alix antibodies were gift from Dr, ScN2a cells infected with 22L prion strain Laude H., Institut National de la Recherche Agronomique

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