 |
Journal articles
Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection.: Salivation and CHIKV
Abstract : BACKGROUND: Aedes aegypti and Aedes albopictus are potential vectors of chikungunya virus (CHIKV). The recent CHIKV outbreaks were caused by a new variant characterized by a mutation in the E1 glycoprotein gene (E1-226V) which has favored a better transmissibility by Ae. albopictus. As Ae. albopictus tends to replace Ae. aegypti in many regions, one question remained: is Ae. albopictus as efficient as Ae. aegypti to transmit the variant E1-226V of CHIKV? METHODOLOGY AND FINDINGS: We infected orally both species with the variant E1-226V and estimated the infection, the viral dissemination, and the transmission rate by real time RT-PCR. Additionally, we used an in vitro assay to determine the amount of virus delivered by mosquitoes in their saliva. We found that Ae. aegypti as well as Ae. albopictus ensured a high replication of the virus which underwent an efficient dissemination as detectable in the salivary glands at day 2 post-infection (pi). Infectious CHIKV particles were delivered by salivary glands from day 2 with a maximum at day 6 pi for Ae. albopictus (10(3.3) PFU) and day 7 pi for Ae. aegypti (10(2.5) PFU). CONCLUSIONS: Ae. albopictus is slightly more efficient than Ae. aegypti to transmit the variant E1-226V of CHIKV. These results will help to design an efficient vector control to limit transmission as soon as the first human cases are diagnosed.
Cited literature [28 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-00395262
Contributor : Anna-Bella Failloux <>
Submitted on : Monday, June 15, 2009 - 12:28:46 PM
Last modification on : Tuesday, December 8, 2020 - 10:08:10 AM
Long-term archiving on: : Thursday, June 10, 2010 - 10:31:49 PM
Contributor : Anna-Bella Failloux <>
Submitted on : Monday, June 15, 2009 - 12:28:46 PM
Last modification on : Tuesday, December 8, 2020 - 10:08:10 AM
Long-term archiving on: : Thursday, June 10, 2010 - 10:31:49 PM
File
Dubrulle_PLoS_2009.pdf
Publisher files allowed on an open archive