Zinc, a novel structural element found in the family of bacterial adenylate kinases

Philippe Glaser (1) , Elena Presecan (2) , Muriel Delepierre (3) , Witold K. Surewicz , Henry H. Mantsch , Octavian Barzu (1) , Anne-Marie Gilles (1)

1 REG - Régulation de l'Expression Génétique
2 Biochimie des Régulations Cellulaires
3 Résonance Magnétique Nucléaire

The adk gene from Bacillus stearothermophilus was cloned and overexpressed in Escherichia coli under the control of the lac promoter. The primary structure of B. stearothermophilus adenylate kinase exhibited 76% identity with the enzyme from Bacillus subtilis, 60% identity with the enzyme from Lactococcus lactis, and 42% identity with the enzyme from E. coli. The most striking property of the adenylate kinase from B. stearothermophilus is the presence of a structural zinc atom bound to four cysteines in a zinc finger-like fashion

Domains: Life Sciences [q-bio]

Philippe Glaser, Elena Presecan, Muriel Delepierre, Witold K. Surewicz, Henry H. Mantsch, et al.. Zinc, a novel structural element found in the family of bacterial adenylate kinases. biochemistry (Moscow), 1992, 31, pp.3038-3043. ⟨10.1021/bi00127a002⟩. ⟨pasteur-00389761⟩