Sequence of a staphylococcal gene, vat, encoding an acetyltransferase inactivating the A-type compounds of virginiamycin-like antibiotics. - Archive ouverte HAL Access content directly
Journal Articles Gene Year : 1993

Sequence of a staphylococcal gene, vat, encoding an acetyltransferase inactivating the A-type compounds of virginiamycin-like antibiotics.

(1) , (1) , (2) , (2) , (1)
1
2

Abstract

The Staphylococcus aureus plasmids, pIP680 and pIP1156, which confer resistance to A-type compounds of virginiamycin-like antibiotics (Vml: streptogramin A, pristinamycin IIA, virginiamycin M) and to synergistic mixtures of the A and B compounds of Vml antibiotics, were shown to direct the modification of A-type compounds by acetylation. The vat gene, encoding the acetyltransferase modifying A-type compounds, was isolated from plasmid pIP680 and sequenced. This gene potentially encodes a 219-amino-acid (aa) protein, VAT, of 24 330 Da showing at least 38% aa identity with two chloramphenicol acetyltransferases encoded by cat genes isolated from Escherichia coli and Agrobacterium tumefaciens. Resistance to A-type compounds of Vml antibiotics conferred to S. aureus by vat was not expressed in E. coli, although a protein having a M(r) similar to that encoded by this gene was detected in E. coli minicells. The vat gene was detected by the polymerase chain reaction in two chromosomally located staphylococcal conjugative elements and in the conjugative plasmid, pIP1156, conferring resistance to A-type compounds.

Dates and versions

pasteur-00389755 , version 1 (29-05-2009)

Identifiers

Cite

Jeanine Allignet, Véronique Loncle, Catherine Simenel, Muriel Delepierre, Névine El Solh. Sequence of a staphylococcal gene, vat, encoding an acetyltransferase inactivating the A-type compounds of virginiamycin-like antibiotics.. Gene, 1993, 130 (1), pp.91-8. ⟨10.1016/0378-1119(93)90350-C⟩. ⟨pasteur-00389755⟩

Collections

PASTEUR CNRS
25 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More