A b(1-3) glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is an homolog of the yeast BGL2

Isabelle Mouyna (1) , Robert P. Hartland (1) , Thierry Fontaine (1) , Michel Diaquin (1) , Catherine Simenel (2) , Muriel Delepierre (2) , Bernard Henrissat (3) , Jean-Paul Latgé (1)

1 Aspergillus
2 Résonance Magnétique Nucléaire
3 AFMB - Architecture et fonction des macromolécules biologiques

A 1,3-beta-glucanosyltransferase which introduces intrachain 1,6-beta linkages into 1,3-beta-glucan was isolated from the cell wall of Aspergillus fumigatus. The biochemical and molecular characterization of the A. fumigatus transferase showed it was homologous to the Saccharomyces cerevisiae and Candida albicans transferase Bgl2p. A null mutant constructed in A. fumigatus by gene replacement did not show a distinct phenotype from the parental strain. The putative function of this major cell-wall-associated protein is discussed.

Mots clés: Aspergillus fumigatus; BGT1; cell wall

Domaines: Mycologie

Isabelle Mouyna, Robert P. Hartland, Thierry Fontaine, Michel Diaquin, Catherine Simenel, et al.. A b(1-3) glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is an homolog of the yeast BGL2. Microbiology, 1998, 144, pp.3171-3180. ⟨10.1099/00221287-144-11-3171⟩. ⟨pasteur-00372577⟩