Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Virology Année : 2008

Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone.

Résumé

We and others have uncovered the existence of human T-cell lymphotropic virus type 3 (HTLV-3). We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro, and tax/rex as well as minus-strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24(gag) protein is detected in the cell culture supernatant. Transfection of 293T-long terminal repeat (LTR)-green fluorescent protein (GFP) cells with the HTLV-3 clone promotes formation of syncytia, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.

Dates et versions

pasteur-00363856 , version 1 (24-02-2009)

Identifiants

Citer

Sébastien Alain Chevalier, Nga Ling Ko, Sara Calattini, Adeline Mallet, Marie-Christine Prévost, et al.. Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone.. Journal of Virology, 2008, 82 (13), pp.6747-52. ⟨10.1128/JVI.00247-08⟩. ⟨pasteur-00363856⟩
71 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More