Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration.

Denise Guetard (1) , Raffaella Greco (2) , Minerva Cervantes Gonzalez (1) , Susanna Celli (3) , Anna Kostrzak (1, 4) , Pierre Langlade-Demoyen (1) , Francesco Sala (2) , Simon Wain-Hobson (1) , Monica Sala (1)

1 Rétrovirologie Moléculaire
2 Department of Biology
3 Département d'Immunologie - Department of Immunology
4 Laboratory of Structural Genomics

Transgenic tobacco plants expressing a HIV-1 polyepitope associated with hepatitis B (HBV) virus-like particles (VLPs) were previously described. It is demonstrated here that oral administration of these transgenic plants to humanized HSB mice to boost DNA-priming can elicit anti-HIV-1 specific CD8+ T cell activation detectable in mesenteric lymph nodes. Nevertheless, a significant regulatory T cell activation was induced in vivo by the vaccination protocols. The balance between tolerance and immunogenicity remains the main concern in the proof of concept of plant-based vaccine.

Domaines: Vaccinologie

Denise Guetard, Raffaella Greco, Minerva Cervantes Gonzalez, Susanna Celli, Anna Kostrzak, et al.. Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration.. Vaccine, 2008, 26 (35), pp.4477-85. ⟨10.1016/j.vaccine.2008.06.059⟩. ⟨pasteur-00363402⟩