Skip to Main content Skip to Navigation
Journal articles

Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein.

Abstract : Morphogenesis of infectious HIV-1 involves budding of immature virions followed by proteolytic disassembly of the Gag protein shell and subsequent assembly of processed capsid proteins (CA) into the mature HIV-1 core. The dimeric interface between C-terminal domains of CA (C-CA) has been shown to be important for both immature and mature assemblies. We previously reported a CA-binding peptide (CAI) that blocks both assembly steps in vitro. The three-dimensional structure of the C-CA/CAI complex revealed an allosteric effect of CAI that alters the C-CA dimer interface. Based on this structure, we now investigated the phenotypes of mutations in the binding pocket. CA variants carrying mutations Y169A, L211A, or L211S had a reduced affinity for CAI and were unable to form mature-like particles in vitro. These mutations also blocked morphological conversion to mature virions in tissue culture and abolished infectivity. X-ray crystallographic analyses of the variant C-CA domains revealed that these alterations induced the same allosteric change at the dimer interface observed in the C-CA/CAI complex. These results point to a role of key interactions between conserved amino acids in the CAI binding pocket of C-CA in maintaining the correct conformation necessary for mature core assembly.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-00360870
Contributor : Mireille Gau <>
Submitted on : Thursday, February 12, 2009 - 2:20:16 PM
Last modification on : Monday, January 13, 2020 - 5:08:10 PM

Links full text

Identifiers

Collections

Citation

Vanda Bartonova, Sébastien Igonet, Jana Sticht, Bärbel Glass, Anja Habermann, et al.. Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2008, 283 (46), pp.32024-33. ⟨10.1074/jbc.M804230200⟩. ⟨pasteur-00360870⟩

Share

Metrics

Record views

290