Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction.

Leandro F Estrozi (1) , Emmanuelle Neumann (1) , G. Squires (2) , G. Rozas-Dennis (3) , M. Costabel (4) , Félix A. Rey (5) , D. M. A. Guérin (6) , Jorge Navaza (1)

1 IBS - UMR 5075 - Institut de biologie structurale
2 Institut Curie [Paris]
3 Departamento de Biología, Bioquímica y Farmacia
4 Grupo de Biofísica, Departamento de Física
5 Virologie Structurale
6 Unidad de Biofísica (UPV/EHU-CSIC)

The blood-sucking reduviid bug Triatoma infestans, one of the most important vector of American human trypanosomiasis (Chagas disease) is infected by the Triatoma virus (TrV). TrV has been classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work presents the three-dimensional cryo-electron microscopy (cryo-EM) reconstruction of the TrV capsid at about 25 A resolution and its use as a template for phasing the available crystallographic data by the molecular replacement method. The main structural differences between the cryo-EM reconstruction of TrV and other two viruses, one from the same family, the cricket paralysis virus (CrPV) and the human rhinovirus 16 from the Picornaviridae family are presented and discussed.

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Leandro F Estrozi, Emmanuelle Neumann, G. Squires, G. Rozas-Dennis, M. Costabel, et al.. Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction.. Virology, 2008, 375 (1), pp.85-93. ⟨10.1016/j.virol.2007.12.025⟩. ⟨pasteur-00335426⟩